FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28070
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC1034136.84004275026719No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGA583420.783754898468114No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC309311.01888136800855No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC8022.857142857142857No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC7632.718204488778055No Hit
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC3821.3608835055219095No Hit
CAAGCAATCAAGGCCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG3041.0830067687923048No Hit
CTTCAGTGGAACATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA2660.9476309226932668No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGT2300.8193801211257571No Hit
AACTGTAGCTTACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTA1340.47737798361239764No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGATGCACAATCTCGT930.33131457071606696No Hit
CTTCTTGTTTTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAG910.3241895261845386No Hit
CAACCAAAAGAACAGCCTGTCCCTTATACACATCTCCGAGCCCACGAGAC820.29212682579266125No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCTCACGAGAC750.26718916993231207No Hit
GTAGTGCTGCTCATCGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG710.25293908086925543No Hit
CTTCAGTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA650.23156394727467047No Hit
ACACCAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACG620.220876380477378No Hit
CCTGACTCACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCG520.18525115781973636No Hit
CAACCAAAAGAACAGCCTGTCTTTATACACATCTCCGAGCCCACGAGACG490.1745635910224439No Hit
ACATCAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACG460.1638760242251514No Hit
CAACCAAAAGAACAGCCTGTCTCTATACACATCTCCGAGCCCACGAGACG430.15318845742785892No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG410.14606341289633062No Hit
CAACCAAAAGAACAGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC410.14606341289633062No Hit
CAAGCAATCAAGGCCAGTCTTTATACACATCTCCGAGCCCACGAGACGAT400.14250089063056642No Hit
CAAGCAATCAAGGCCAGTCTGTCTCTTATACACATCTCCGAGCCCACGAG400.14250089063056642No Hit
CAACCAAAAGAACAGCCTGTCTCTTATATACATCTCCGAGCCCACGAGAC390.13893836836480228No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCG380.1353758460990381No Hit
CAAGCAATCAAAGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGA370.13181332383327396No Hit
CTTCAGTGGAGCATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA370.13181332383327396No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACATATCTCCGAGCCCACGAGAC360.12825080156750981No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATTCCGAGCCCACGAGACG350.12468827930174563No Hit
CCTGACTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGT340.12112575703598148No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG340.12112575703598148No Hit
CAACCAAAAGAACAGCCTGTCTCTTTACACATCTCCGAGCCCACGAGACG340.12112575703598148No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACAAATCTCCGAGCCCACGAGAC340.12112575703598148No Hit
CAAGCAATCAAGGCCAGTCCCTTATACACATCTCCGAGCCCACGAGACGA320.11400071250445315No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCACACGAGAC310.110438190238689No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTTCGAGCCCACGAGAC310.110438190238689No Hit
CAACCAAAAGAACAGCCTGTCTCTTTTACACATCTCCGAGCCCACGAGAC310.110438190238689No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC310.110438190238689No Hit
CCTTTAACAAGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC300.10687566797292483No Hit
CAAGCAAAAGAACAGCCTGTCCCTTATACACATCTCCGAGCCCACGAGAC300.10687566797292483No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCCCCGAGCCCACGAGACGA300.10687566797292483No Hit
CAACCAAAAGAACAGCCTGTCTCTGATACACATCTCCGAGCCCACGAGAC290.10331314570716067No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)