FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5909
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC177430.022000338466746No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCC112819.089524454222374No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC5619.49399221526485No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC1342.2677271958030123No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC1181.99695379928922No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCCACGTATATCTCGT881.4892536808258587No Hit
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC731.2354036215941784No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC641.08309358605517No Hit
CAAGCAATCAAGGCCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG631.066170248773058No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG621.049246911490946No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG560.9477068877982738No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG470.7953968522592656No Hit
CTTCAGTGGAACATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA370.6261634794381452No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGT360.6092401421560332No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT330.558470130309697No Hit
AACTGTAGCTTACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTA330.558470130309697No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT320.541546793027585No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC300.5077001184633609No Hit
GTAGTGCTGCTCATCGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG290.4907767811812489No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC250.4230834320528008No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC220.3723134202064647No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT210.3553900829243527No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC200.33846674564224066No Hit
CTTCTTGTTTTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAG190.3215434083601286No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA170.28769673379590455No Hit
ACACCAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACG160.2707733965137925No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG150.25385005923168047No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT140.23692672194956846No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCACACGAGAC140.23692672194956846No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG120.2030800473853444No Hit
CCTGACTCACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCG120.2030800473853444No Hit
CTTCAGTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA120.2030800473853444No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACATATCTCCGAGCCCACGAGAC120.2030800473853444No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG110.18615671010323234No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCTCACGAGAC110.18615671010323234No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT110.18615671010323234No Hit
CAAGCAATCAAAGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCC110.18615671010323234No Hit
CAACCAAAAGAACAGCCTGTCTTTATACACATCTCCGAGCCCACGAGACC100.16923337282112033No Hit
GTCATTGTCACTGGTCAGCTCCGAGCCCACGAGACCCACGTATATCTCGT100.16923337282112033No Hit
CAACCAAAAGAACAGCCTGTCTCTTATATACATCTCCGAGCCCACGAGAC100.16923337282112033No Hit
CAAGCAATCAAGGCCAGTCTGTCTCTTATACACATCTCCGAGCCCACGAG100.16923337282112033No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGGACC90.1523100355390083No Hit
CAACCAAAAGAACAGCCTGTCCCTTATACACATCTCCGAGCCCACGAGAC90.1523100355390083No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACAACCAACGATCTCGT90.1523100355390083No Hit
GAAGCAGTGGATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCG90.1523100355390083No Hit
ACATCAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACG90.1523100355390083No Hit
CAAGCAATCAAGGCCAGTCTTTATACACATCTCCGAGCCCACGAGACCCA90.1523100355390083No Hit
CAACCAAAAGAACAGCCTGTCTCTATACACATCTCCGAGCCCACGAGACC90.1523100355390083No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCCCCGAGCCCACGAGACCC80.13538669825689625No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG80.13538669825689625No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCACACGAGACCC80.13538669825689625No Hit
CAACCAAAAGAACAGCCTGTCTCTTATAACATCTCCGAGCCCACGAGACC80.13538669825689625No Hit
GTGGCTGCTCATCGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC70.11846336097478423No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCATGAGAC70.11846336097478423No Hit
CCTGACTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGT70.11846336097478423No Hit
CAAGCAATCAAGGCCAGTCTCTTATACAAATCTCCGAGCCCACGAGACCC70.11846336097478423No Hit
CAACCAAAAGAACAGCCTGTCTCTTATCACATCTCCGAGCCCACGAGACC70.11846336097478423No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACAGACC70.11846336097478423No Hit
CAAGCAAAAGAACAGCCTGTCCCTTATACACATCTCCGAGCCCACGAGAC60.1015400236926722No Hit
CAATCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC60.1015400236926722No Hit
CAAGCAATCAAGGCCAGTCCCTTATACACATCTCCGAGCCCACGAGACCC60.1015400236926722No Hit
CTTCAGTGGAGCATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA60.1015400236926722No Hit
CAACCAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACC60.1015400236926722No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAC60.1015400236926722No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCTCACGAGACCC60.1015400236926722No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATTTCCGAGCCCACGAGAC60.1015400236926722No Hit
CAACCAAAAGAACAGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC60.1015400236926722No Hit
CAACCAAAAGAACAGCCTGTCTCTGATACACATCTCCGAGCCCACGAGAC60.1015400236926722No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGTCCACGAGAC60.1015400236926722No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCAGAGACCCA60.1015400236926722No Hit
CAAGCAATCAAGGCCAGTCTCTGATACACATCTCCGAGCCCACGAGACCC60.1015400236926722No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCAAGAGAC60.1015400236926722No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)