FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156941
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGC4840.30839614887123185No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACG4010.2555100324325702No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG3360.2140931942577147No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT2890.1841456343466653No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACG2500.159295531441752No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG1950.12425051452456656Illumina PCR Primer Index 11 (96% over 29bp)
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACT1730.11023250775769237No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT1660.10577223287732333No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)