FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002294877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342840
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG39921.164391552910979RNA PCR Primer, Index 27 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG21110.6157391202893479RNA PCR Primer, Index 27 (96% over 30bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGC20250.5906545327266364No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG12660.36926846342317116RNA PCR Primer, Index 27 (96% over 30bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG12550.3660599696651499RNA PCR Primer, Index 27 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG12420.3622681134056703RNA PCR Primer, Index 27 (96% over 30bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC10530.3071403570178509RNA PCR Primer, Index 27 (95% over 21bp)
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG9040.26367985065919963RNA PCR Primer, Index 27 (100% over 28bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC5700.1662583129156458RNA PCR Primer, Index 27 (95% over 21bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC5600.16334150040835377RNA PCR Primer, Index 27 (95% over 21bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC5390.1572161941430405RNA PCR Primer, Index 27 (95% over 21bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGC4300.12542293781355734No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTA3720.10850542527126356No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCC3470.1012133940030335No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)