FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002294922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGA17.142857142857142No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCCTGT17.142857142857142No Hit
GCGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCT17.142857142857142No Hit
GATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCA17.142857142857142No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGA17.142857142857142No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG17.142857142857142TruSeq Adapter, Index 8 (96% over 29bp)
CTGGAGGCAAGTAGACTTTCCCGATCGATTTTGTTTTCTACTCCGATTGCAGATGATGTTATCGCTTTGTCGGGG17.142857142857142No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACACTGTCTCTTATACACATCTCCGAGCCCAC17.142857142857142No Hit
GTGGTATCAACGCAGAGTACATGGGAAGGGGTGCGCCCCTGTAGCTGTCTCTTATACACATCTCCGAGCCCACGA17.142857142857142No Hit
AGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCG17.142857142857142No Hit
AGCGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG17.142857142857142No Hit
CAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCAGAGCCCCTGTCTCTTATACACATCTCCGA17.142857142857142No Hit
GTATCATGAATTTGTATTCTCTGGCTGGACCGCTTTATAGCGGTGCCGGGTCATTGGCCTCTCACCTATCAACTC17.142857142857142No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCT17.142857142857142No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)