FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002294990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1765433
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGC271971.540528584205688No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGC133320.7551688452634566No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG111470.6314031741787992RNA PCR Primer, Index 45 (96% over 30bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG52280.2961313173595373No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGC49590.2808942622008312No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGC48710.27590964936080836No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG46300.2622586073784732RNA PCR Primer, Index 45 (96% over 30bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCAT45840.2596530143030067No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTC44690.2531390316143405RNA PCR Primer, Index 45 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG44180.2502502219002364RNA PCR Primer, Index 45 (96% over 30bp)
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCAT40130.22730967417058592No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATG34940.1979117870799968No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTC34140.19338032086179424RNA PCR Primer, Index 45 (95% over 21bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTC30840.1746880227117087RNA PCR Primer, Index 45 (95% over 21bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA29140.16505865699802824No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG28870.1635292871493849No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGC28820.16324607051074722No Hit
ATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG28560.1617733439898314RNA PCR Primer, Index 45 (96% over 30bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCAT27210.15412649474661458No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTC26570.1505013217720525RNA PCR Primer, Index 45 (95% over 21bp)
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGC24260.13741671306699263No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATG23510.13316846348742772No Hit
CATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG21850.12376567108465743RNA PCR Primer, Index 45 (96% over 30bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCC20860.11815798163963176No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGA20860.11815798163963176RNA PCR Primer, Index 45 (96% over 30bp)
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATT20400.11555238856416528No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCAT20280.1148726686314349No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA19870.11255029219460608No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACG19760.11192721558960324No Hit
CACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG18450.10450693965729654RNA PCR Primer, Index 45 (100% over 29bp)
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTC17680.10014540342227658RNA PCR Primer, Index 45 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)