FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002294993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1901477
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGC188280.9901776355959078No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGC92890.48851498072288013No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG74320.3908540571355846Illumina PCR Primer Index 7 (96% over 27bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG37560.19753065643181592No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAT32790.1724448941533345No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC32070.168658363998092No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCC31790.1671858244932755No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAT31630.16634437334766605No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG29610.15572105263434688TruSeq Adapter, Index 18 (96% over 29bp)
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCC28760.15125084342329673No Hit
ACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG28630.15056716436748907TruSeq Adapter, Index 18 (96% over 29bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC27740.14588659237003654No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC27350.14383555520261354No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC25920.13631508558872918No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATG21020.11054564425444009No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG19750.10386662578616516No Hit
ATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG19330.10165781652894039Illumina PCR Primer Index 7 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)