FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1936
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG1115.733471074380166No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT1055.4235537190082646No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG1025.268595041322314No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG1005.1652892561983474No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC954.90702479338843No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT884.545454545454546No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT874.493801652892563No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC743.8223140495867765No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAG703.6157024793388426No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA653.3574380165289255No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA633.254132231404959No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC603.0991735537190084No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC582.9958677685950414No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG562.8925619834710745No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG522.6859504132231407No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG472.427685950413223No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG442.272727272727273No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG412.1177685950413223No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC351.8078512396694213No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT351.8078512396694213No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC331.7045454545454544No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC321.6528925619834711No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATT291.4979338842975207No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTT281.4462809917355373No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAC251.2913223140495869No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT231.18801652892562No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATA221.1363636363636365No Hit
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC201.0330578512396695No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT201.0330578512396695No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAAACCA140.7231404958677686No Hit
CCTGACTCACGTTTCCTTATTGCTGGGATGTTCACAGAGCCAACGTCCTG140.7231404958677686No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAACCAC110.5681818181818182No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCT100.5165289256198348No Hit
CTGTTTTCATCCCACAGGTTGTTATTTTGTACTTTGAGCCAAGCACATTT80.4132231404958678No Hit
CTTCAGTGGAACATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA80.4132231404958678No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGACACCGGGTCATTCTTTT60.30991735537190085No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACAACCAACGATCTCGT40.2066115702479339No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC40.2066115702479339No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGGCACCGGGTCATTCTTTT40.2066115702479339No Hit
CCTGACTCACGTTTCCTTATTGCTGGGATGTTCACAGAGCCAACGTCTTG30.15495867768595042No Hit
TTTCAGTTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC30.15495867768595042No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCCCT30.15495867768595042No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCCACGTATATCTCGT30.15495867768595042No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGT30.15495867768595042No Hit
CAAGCAATCAAGGCCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG20.10330578512396695No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCG20.10330578512396695No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACACCATTCAATCTCGT20.10330578512396695No Hit
CAACCAAAAGAACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC20.10330578512396695No Hit
CCTGACTCACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCG20.10330578512396695RNA PCR Primer, Index 32 (95% over 22bp)
GGTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTG20.10330578512396695No Hit
CCTTTAACAAGTATGCCTGTCAGCATCACACTGGTGGCCACTTCTACAAT20.10330578512396695No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC20.10330578512396695No Hit
GACACTTGGCCTGGAGTACGGTGCTGGCGTTGAGGCTGTGGTAGGGCCGG20.10330578512396695No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCACACGAGACAC20.10330578512396695No Hit
TTTCAGGTAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCC20.10330578512396695No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)