FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC1117.628865979381444No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG805.498281786941581No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACAACCAACGATCTCGT724.948453608247423No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC634.329896907216495No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG594.054982817869416No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT533.6426116838487976No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG523.5738831615120272No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT402.7491408934707904No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGTTGGAAGATCTCGT362.4742268041237114No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC362.4742268041237114No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG342.336769759450172No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG302.0618556701030926No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA271.8556701030927836No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG251.718213058419244No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC241.6494845360824744No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT241.6494845360824744No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC231.5807560137457044No Hit
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC221.5120274914089347No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG221.5120274914089347No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAG201.3745704467353952No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT191.3058419243986255No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG181.2371134020618557No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC181.2371134020618557No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCG171.168384879725086No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTT171.168384879725086No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACATCATCTCATCTCGT161.0996563573883162No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG161.0996563573883162No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT151.0309278350515463No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATA140.9621993127147767No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCATCGACCATCTCGT130.8934707903780068No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCG130.8934707903780068No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGATGCACAATCTCGT130.8934707903780068No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA130.8934707903780068No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGG120.8247422680412372No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATT100.6872852233676976No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC100.6872852233676976No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT80.5498281786941581No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCT70.48109965635738833No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACAACCAACGAACTCGT70.48109965635738833No Hit
GTCATTGTCACTGGTCAGCTCCGAGCCCACGAGACAACCAACGATCTCGT70.48109965635738833No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCG60.4123711340206186No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACAACCAACGACCTCGT50.3436426116838488No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCG50.3436426116838488No Hit
TTTCAGGTCAGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCT50.3436426116838488RNA PCR Primer, Index 37 (95% over 24bp)
CCTGACTCACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCG40.27491408934707906No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGTTGGAAGACCTCGT40.27491408934707906No Hit
CTGTTTTCATCCCACAGGTTGTTATTTTGTACTTTGAGCCAAGCACATTT40.27491408934707906No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGTTGGAAGAACTCGT40.27491408934707906No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGGCACCGGGTCATTCTTTT40.27491408934707906No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCCCCGGGGCGGCAGGTGGGGCG30.2061855670103093No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCATCGACCACCTCGT30.2061855670103093No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGTGCATTCATCTCGT30.2061855670103093No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACCATCGACCACCTCGTATGCCG30.2061855670103093No Hit
GTCATTGTCACTGGTCAGCTCCGAGCCCACGAGACGTTGGAAGATCTCGT30.2061855670103093No Hit
GTTTCCAGGTCAAGGGTAACGGAACGTGGACCAAGTCCCGCGTCTCCTCC30.2061855670103093No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCG30.2061855670103093No Hit
GTCATTGTCACTGGTCAGCTCCGAGCCCACGAGACAACCAACGAACTCGT30.2061855670103093No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCG30.2061855670103093No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACAACCAACGACCTCGTATGCCG30.2061855670103093No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGACACCGGGTCATTCTTTT30.2061855670103093No Hit
CTTCAGTGGAACATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA30.2061855670103093No Hit
GTAAAAGTTGAAATGTCAGGGCCCACGAGACGATGCACAATCTCGTATGC20.13745704467353953No Hit
AACTGTAGCTTACACTAACTCCGAGCCCACGAGACGTTGGAAGATCTCGT20.13745704467353953No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACAACCAACGAGCTCGT20.13745704467353953No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGATCG20.13745704467353953No Hit
GTCATTGTCACTGGTCAGCTCCGAGCCCACGAGACATCATCTCATCTCGT20.13745704467353953No Hit
GTCATTGTCACTGGTCAGCTCCGAGCCCACGAGACCATCGACCATCTCGT20.13745704467353953No Hit
AACTGTAGCTTACACTGACCCCGAGCCCACGAGACAACCAACGATCTCGT20.13745704467353953No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACAACCAACGATATCGT20.13745704467353953No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGTGCATTCAACTCGT20.13745704467353953No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCCACGTATATCTCGT20.13745704467353953No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAAACCA20.13745704467353953No Hit
CCTGACTCACGTTTCCTTATTGCTGGGATGTTCACAGAGCCAACGTCCTG20.13745704467353953No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACACCTCCGAGCCCACGAGACGG20.13745704467353953No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGTTGGAAGAGCTCGT20.13745704467353953No Hit
CCTGACTCACATTTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCG20.13745704467353953No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)