FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28563
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC2196176.88618142351994No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC12414.344781710604629No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGA6812.3842033399852953No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC3421.1973532191996639No Hit
GGTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTG2600.9102685292161187No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCCCCACGTTGC960.33609914924902845No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC880.30809088681160945No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACAACCAACGATCTCGT760.2660784931554809No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG700.2450722963274166No Hit
GTCATTGTCACTGGTCAGCTCCAACACCTTCTGCTGCGTCTCCACGTTGC670.23456919791338443No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC590.20656093547596543No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG570.19955886986661067No Hit
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC470.16454854181983683No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT440.15404544340580473No Hit
GTCATTGTCACTGGTCAGCCCCAGCACCTTCTGCTGCGTCTCCACGTTGC380.13303924657774044No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC370.12953821377306307No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGTGTCTCCACGTTGC370.12953821377306307No Hit
GTATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCG360.12603718096838568No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA360.12603718096838568No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT350.1225361481637083No Hit
GTCATTGTACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCG350.1225361481637083No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGTTGGAAGATCTCGT330.11553408255435353No Hit
TCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCG320.11203304974967615No Hit
GTCATTATCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC320.11203304974967615No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCTACGTTGC310.10853201694499878No Hit
GTCATTGTCACTGATCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC310.10853201694499878No Hit
GTCATGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCG300.1050309841403214No Hit
GTCATTGTCACTGGTCAACTCCAGCACCTTCTGCTGCGTCTCCACGTTGC300.1050309841403214No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG300.1050309841403214No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)