FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4404
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC145733.08356039963669No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCA68815.622161671207992No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC3768.537693006357856No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC1312.974568574023615No Hit
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC912.0663033605812897No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC761.725703905540418No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG641.4532243415077202No Hit
CTTCAGTGGAACATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA481.08991825613079No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACAACCAACGATCTCGT481.08991825613079No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCATCGACCATCTCGT430.9763851044504995No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG430.9763851044504995No Hit
CAAGCAATCAAGGCCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG350.7947320617620345No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC290.6584922797456858No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGT270.6130790190735695No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG260.5903723887375113No Hit
CCTGACTCACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG240.544959128065395No Hit
GTAGTGCTGCTCATCGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG210.4768392370572207No Hit
AACTGTAGCTTACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTA170.38601271571298823No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGTTGGAAGATCTCGT170.38601271571298823No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC140.31789282470481384No Hit
CAACCAAAAGAACAGCCTGTCCCTTATACACATCTCCGAGCCCACGAGAC130.29518619436875565No Hit
CTTCAGTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA120.2724795640326975No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG110.24977293369663942No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC100.2270663033605813No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT100.2270663033605813No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT100.2270663033605813No Hit
CAACCAAAAGAACAGCCTGTCTCTTATATACATCTCCGAGCCCACGAGAC100.2270663033605813No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCG100.2270663033605813No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC90.20435967302452315No Hit
CTTCTTGTTTTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAG90.20435967302452315No Hit
CTTCAGTGGAGCATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA90.20435967302452315No Hit
ACACCAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACG90.20435967302452315No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC90.20435967302452315No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCTCACGAGAC90.20435967302452315No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCACACGAGAC90.20435967302452315No Hit
CAACCAAAAGAACAGCCTGTCTCTTATAACATCTCCGAGCCCACGAGACC90.20435967302452315No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA80.18165304268846502No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATTTCCGAGCCCACGAGAC80.18165304268846502No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACATATCTCCGAGCCCACGAGAC80.18165304268846502No Hit
CAAGCAATCAAGGCCAGTCTCTTATACATATCTCCGAGCCCACGAGACCA70.15894641235240692No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGGACC70.15894641235240692No Hit
CAACCAAAAGAACAGCCTGTCTCTGATACACATCTCCGAGCCCACGAGAC70.15894641235240692No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACAAATCTCCGAGCCCACGAGAC70.15894641235240692No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCG60.13623978201634876No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCCCCGAGCCCACGAGACCA60.13623978201634876No Hit
CAATCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC60.13623978201634876No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG60.13623978201634876No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCATGAGAC60.13623978201634876No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACGTCTCCGAGCCCACGAGAC60.13623978201634876No Hit
CAACCAAAAGAACAGCCTGTCTCTATACACATCTCCGAGCCCACGAGACC60.13623978201634876No Hit
CAAGCAATCAAAGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCA60.13623978201634876No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCACACGAGAC50.11353315168029066No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGATCA50.11353315168029066No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACAATCTCCGAGCCCACGAGACC50.11353315168029066No Hit
CAACCAAAAGAACAGCCTGTCTTTATACACATCTCCGAGCCCACGAGACC50.11353315168029066No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCGAGCCCACGAGACC50.11353315168029066No Hit
CAACCAAAAGAACAGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC50.11353315168029066No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCG50.11353315168029066No Hit
GTCATTGTCACTGGTCAGCTCCGAGCCCACGAGACCATCGACCATCTCGT50.11353315168029066No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTTCGAGCCCACGAGAC50.11353315168029066No Hit
CCTTTAACAAGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC50.11353315168029066No Hit
CAACCAAAAGAACAGCCTGTCTCTTAACACATCTCCGAGCCCACGAGACC50.11353315168029066No Hit
CAAGCAATCAAGGCCAGTCTGTCTCTTATACACATCTCCGAGCCCACGAG50.11353315168029066No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCTGAGCCCACGAGAC50.11353315168029066No Hit
CAACCAAAAGAACAGCCTGTCTTTTATACACATCTCCGAGCCCACGAGAC50.11353315168029066No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCAAGAGACCA50.11353315168029066No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)