FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3191
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC93429.269821372610465No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGT44113.820119084926356No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC2638.241930429332497No Hit
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC1153.603885929175807No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC642.005640864932623No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG621.9429645879034785No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC471.472892510184895No Hit
AACTGTAGCTTACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTA421.3162018176120338No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG351.0968348480100283No Hit
CTTCAGTGGAACATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA341.065496709495456No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGT341.065496709495456No Hit
CCTGACTCACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCG331.0341585709808836No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG321.0028204324663115No Hit
CAAGCAATCAAGGCCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG270.8461297398934503No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC220.6894390473205891No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT200.6267627702914447No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC190.5954246317768724No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG170.532748354747728No Hit
CTTCAGTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA150.4700720777185835No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC140.43873393920401127No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT140.43873393920401127No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT140.43873393920401127No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG130.40739580068943904No Hit
CAACCAAAAGAACAGCCTGTCTTTATACACATCTCCGAGCCCACGAGACG130.40739580068943904No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA130.40739580068943904No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG120.3760576621748668No Hit
GTAGTGCTGCTCATCGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG120.3760576621748668No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC120.3760576621748668No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGTTGGAAGATCTCGT110.34471952366029457No Hit
CTTCTTGTTTTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAG110.34471952366029457No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACAACCAACGATCTCGT110.34471952366029457No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCG100.31338138514572234No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC90.2820432466311501No Hit
GGCTGTGGTGTGAGTCTGGGACTTGAACTCTGTCTCTTATACACATCTCC90.2820432466311501No Hit
CAACCAAAAGAACAGCCTGTCCCTTATACACATCTCCGAGCCCACGAGAC90.2820432466311501No Hit
ACACCAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACG90.2820432466311501No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATT90.2820432466311501No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG80.25070510811657787No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT80.25070510811657787No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG80.25070510811657787No Hit
CAAGCAATCAAGGCCAGTCTGTCTCTTATACACATCTCCGAGCCCACGAG80.25070510811657787No Hit
CAACCAAAAGAACAGCCTGTCTTTTATACACATCTCCGAGCCCACGAGAC80.25070510811657787No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCTCACGAGAC70.21936696960200563No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCG70.21936696960200563No Hit
ACATCAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACG70.21936696960200563No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTT70.21936696960200563No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATTCCGAGCCCACGAGACG60.1880288310874334No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA60.1880288310874334No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACAATCTCCGAGCCCACGAGACG60.1880288310874334No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACATATCTCCGAGCCCACGAGAC60.1880288310874334No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACAAATCTCCGAGCCCACGAGAC60.1880288310874334No Hit
CAATCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC50.15669069257286117No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAACCAC50.15669069257286117No Hit
CCTGACTCACGTTTCCTTATACACATCTCCGAGCCCACGAGACGTTGGAA50.15669069257286117No Hit
CAACCAAAAGAACAGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC50.15669069257286117No Hit
CAAGCAATCAAAGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGT50.15669069257286117No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAG50.15669069257286117No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT50.15669069257286117No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATA50.15669069257286117No Hit
CAAGCAATCAAGGCCAGTCTCTTAACACATCTCCGAGCCCACGAGACGTT40.12535255405828893No Hit
CTGTTTTCATCCCACAGGTTGTTATTTTGTACTTTGAGCCAAGCACATTT40.12535255405828893No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACCTCTCCGAGCCCACGAGACGT40.12535255405828893No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACCATCTCCGAGCCCACGAGACG40.12535255405828893No Hit
CAACCAAAAGAACAGCCTGTCTATTATACACATCTCCGAGCCCACGAGAC40.12535255405828893No Hit
CCTGACTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGT40.12535255405828893No Hit
CAAGCAATCAAGGCCAGTCTTTTATACACATCTCCGAGCCCACGAGACGT40.12535255405828893No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGATGCACAATCTCGT40.12535255405828893No Hit
CAAGCAATCAAGGCCAGTCTCTTATACAAATCTCCGAGCCCACGAGACGT40.12535255405828893No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGACACCGGGTCATTCTTTT40.12535255405828893No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)