FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12122
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC398432.86586371885827No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAA212617.53836000659957No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC10768.876423032502888No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC3202.639828411153275No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC2722.2438541494802835No Hit
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC1791.4766540174888634No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACAACCAACGATCTCGT1271.047681900676456No Hit
CTTCAGTGGAACATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA1090.8991915525490843No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC1070.8826926249793763No Hit
CAAGCAATCAAGGCCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG1010.8331958422702525No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG1010.8331958422702525No Hit
AACTGTAGCTTACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTA990.8166969147005444No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG900.7424517406368586No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGT860.7094538854974426No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC760.6269592476489028No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT730.6022108562943409No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG660.5444646098003629No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT640.527965682230655No Hit
CCTGACTCACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCG620.511466754660947No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT550.4537205081669692No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT530.4372215805972612No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG460.37947533410328327No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT390.3217290876093054No Hit
CTTCTTGTTTTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAG340.28048176868503544No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA340.28048176868503544No Hit
CTTCAGTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA330.27223230490018147No Hit
ACACCAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACG330.27223230490018147No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG310.2557333773304735No Hit
CAACCAAAAGAACAGCCTGTCCCTTATACACATCTCCGAGCCCACGAGAC300.24748391354561952No Hit
GTAGTGCTGCTCATCGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG280.23098498597591155No Hit
CAACCAAAAGAACAGCCTGTCTTTATACACATCTCCGAGCCCACGAGACA270.22273552219105758No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCTCACGAGAC270.22273552219105758No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC270.22273552219105758No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG250.2062365946213496No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC240.1979871308364956No Hit
CTTCAGTGGAGCATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA240.1979871308364956No Hit
GAAGCAGTGGATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCG210.17323873948193366No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC200.1649892756970797No Hit
GGCTGTGGTGTGAGTCTGGGACTTGAACTCTGTCTCTTATACACATCTCC180.14849034812737172No Hit
CAAGCAATCAAGGCCAGTCTTTATACACATCTCCGAGCCCACGAGACAAC180.14849034812737172No Hit
CAACCAAAAGAACAGCCTGTCTCTTATATACATCTCCGAGCCCACGAGAC180.14849034812737172No Hit
CAAGCAATCAAGGCCAGTCTCTTATACAAATCTCCGAGCCCACGAGACAA170.14024088434251772No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG170.14024088434251772No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACAAATCTCCGAGCCCACGAGAC170.14024088434251772No Hit
CAAGCAATCAAGGCCAGTCTGTCTCTTATACACATCTCCGAGCCCACGAG170.14024088434251772No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATTCCGAGCCCACGAGACA160.13199142055766375No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACAATCTCCGAGCCCACGAGACA150.12374195677280976No Hit
GTGGCTGCTCATCGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC150.12374195677280976No Hit
CAACCAAAAGAACAGCCTGTCTCTTGTACACATCTCCGAGCCCACGAGAC140.11549249298795577No Hit
CCTGACTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGT140.11549249298795577No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCGAGCCCACGAGACA140.11549249298795577No Hit
CAACCAAAAGAACAGCCTGTCTCTGATACACATCTCCGAGCCCACGAGAC140.11549249298795577No Hit
CAACCAAAAGAACAGCCTGTCTCTTTTACACATCTCCGAGCCCACGAGAC140.11549249298795577No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA130.10724302920310179No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCG130.10724302920310179No Hit
CAACCAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACA130.10724302920310179No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)