FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002295044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190325
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG49080.4123243651943797TruSeq Adapter, Index 8 (96% over 29bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGC23230.19515678491168378No Hit
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG21970.18457144057295277RNA PCR Primer, Index 8 (96% over 29bp)
CTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG21760.1828072165164976RNA PCR Primer, Index 8 (96% over 29bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC19190.1612164744922605No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC15810.1328208682502678No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG15570.1308046121857476TruSeq Adapter, Index 8 (96% over 29bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC15270.12828429210509734No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)