FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002295077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2001241
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGC148530.7421894714329759No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC45530.2275088307705069No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG41590.20782104704031149TruSeq Adapter, Index 7 (96% over 28bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGC38200.19088155799326517No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATC35900.179388689318278No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG31480.157302393864607No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGAC27610.13796439309408512No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGA27270.1362654472899566No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCA26830.1340668115434373No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC25760.1287201291598563No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC24400.12192434594334214No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATC22110.11048144626259405No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)