FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002295093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466026
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC28995419.7782303997337No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC1282268.746502449479069RNA PCR Primer, Index 32 (95% over 24bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC1214798.286278688099666RNA PCR Primer, Index 32 (95% over 24bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC1185448.086077600260841No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC1035527.063449079347843RNA PCR Primer, Index 32 (95% over 24bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC664264.531024688511664RNA PCR Primer, Index 32 (95% over 24bp)
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTC456003.110449610034201No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAA377382.574169898760322No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC153031.043842332946346RNA PCR Primer, Index 32 (95% over 24bp)
GGTCGGTAGTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGC137120.9353176546664248RNA PCR Primer, Index 32 (96% over 30bp)
CCCGATGAGCAGCCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTT97990.6684056080860776RNA PCR Primer, Index 32 (96% over 26bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGC90560.6177243786945116No Hit
GTCCAGGGTGGACATGGGCACACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC88470.6034681513151882No Hit
CTAGTTTCCTCTGGCTTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC80940.5521048057810707RNA PCR Primer, Index 32 (95% over 24bp)
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATC73380.5005368254041879No Hit
CTGCTTCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTG73330.5001957673329122RNA PCR Primer, Index 32 (96% over 33bp)
GTCCAGGGTGGGCATGGGCACACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC58560.39944721307807635No Hit
GCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC56130.3828717908140783RNA PCR Primer, Index 32 (95% over 24bp)
CATCCGCAGCGTCCTCATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGT55160.3762552642313301RNA PCR Primer, Index 32 (95% over 23bp)
CATCCATAGCGTCCTCATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGT51840.35360900829862496RNA PCR Primer, Index 32 (95% over 23bp)
CCCGATGAGCAGCACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC51330.35013021597161303RNA PCR Primer, Index 32 (95% over 24bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCCGTC48060.32782501811018355No Hit
TCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC47520.32414159094040623RNA PCR Primer, Index 32 (95% over 24bp)
GCTCTCAGCTCCGTAGTGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCG47430.32352768641211005RNA PCR Primer, Index 32 (95% over 22bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCCGTC44220.30163175823621136No Hit
GTTTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTAT38570.26309219618205953No Hit
GGTCATTGTCACTGGTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGT38220.26070478968312977RNA PCR Primer, Index 32 (95% over 23bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGC36980.2522465495154929No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCCGTC35760.24392473257636632No Hit
CATCCGCAGCGTCCTCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC35200.24010488217807868RNA PCR Primer, Index 32 (95% over 24bp)
AAAGTTGAAATGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTC34810.23744462922212836No Hit
CTTCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC34670.23648966662255647RNA PCR Primer, Index 32 (95% over 24bp)
CCCGATGAGCAGCCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGT34350.23430689496639215RNA PCR Primer, Index 32 (95% over 23bp)
GTTTCCAGGTCAAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATC33680.22973671681129804No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCCGTC33070.22557580834173474No Hit
CAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTG32350.22066457211536494RNA PCR Primer, Index 32 (96% over 33bp)
GTTTCCACGTCAGAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCG31290.2134341410043205RNA PCR Primer, Index 32 (95% over 22bp)
CATCCATAGCGTCCTCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC31050.21179706226219724RNA PCR Primer, Index 32 (95% over 24bp)
CTTCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC30560.2084546931636956RNA PCR Primer, Index 32 (95% over 24bp)
GCTCTCAGCTCCGTAAAGGTGGAGCTCATCTTCATTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCA26850.18314818427504015No Hit
CTAGTTTCCTTTGGCTTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC24380.16629991555402154RNA PCR Primer, Index 32 (95% over 24bp)
GTTTCCAGGTCAGAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCG24030.16391250905509178RNA PCR Primer, Index 32 (95% over 22bp)
GTTTCCAGGTCAAGGGTAACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAA22790.15545426888745492No Hit
CATCCGCAGCGTCATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC22320.15224832301746355RNA PCR Primer, Index 32 (95% over 24bp)
AAAGTTGAAATGTCAGGGTCATGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTC22050.15040660943257486No Hit
CATCCGCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTG19380.13219410842645354RNA PCR Primer, Index 32 (96% over 33bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAAGCTCGTATGC19080.13014775999879946No Hit
GTCCAGGGTGGAGCTCATCTTCATTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGT18850.12857889287093133No Hit
AAAGTTGAAATATCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTC18500.1261914863720016No Hit
CATCCGTAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC17680.12059813400308043RNA PCR Primer, Index 32 (95% over 24bp)
GGCTGTGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC16870.11507299324841443No Hit
GGCTGTGGTGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGC16350.11152598930714736RNA PCR Primer, Index 32 (96% over 30bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATGTCGTATGC15910.10852467827992136No Hit
CCCGATGAGCAGCCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCCGTCTT15330.10456840465312349No Hit
GACCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC15100.10299953752525536No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)