FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002295094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231566
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG16110.6956979867510774Illumina PCR Primer Index 10 (96% over 27bp)
CTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG5110.22067142844804502Illumina PCR Primer Index 10 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG4530.19562457355570334Illumina PCR Primer Index 10 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG2740.11832479725002808Illumina PCR Primer Index 10 (96% over 27bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGC2710.11702927027283799No Hit
CATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG2390.10321031584947704Illumina PCR Primer Index 10 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)