FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002295111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2701889
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGC652502.414977077148617No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGC198370.7341900425961244No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC163740.6060204545782598No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG122680.45405270164688477No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCT116600.43154992673644255No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCT116430.4309207373063808No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC112630.4168565029873544No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG111160.41141586497446786No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA106770.39516797322169783No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC105380.39002342435236975No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC104480.3866924214873372No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA100550.37214704231002826No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCA95320.3527902145498945No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG83420.30874695444557493RNA PCR Primer, Index 26 (100% over 29bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGC67090.2483077580167061No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA46820.17328617126758353No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACT46010.17028826868905422No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATTC45300.16766047753997296No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGC41790.1546695663663459No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCT40940.15152361921603735No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA37360.13827363004179669No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG37180.13760742946879015No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCT37000.13694122889578367No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGC33700.12472755172399753No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCC32950.12195171600313706No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGG32770.12128551543013055No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAT31260.11569683284546478No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA30320.11221778540865299No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA29890.11062630626202631No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGC29580.10947896083073731No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC29310.10847965997122754No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC28140.10414935624668518No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)