FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16245
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT6243.84118190212373No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT1981.218836565096953RNA PCR Primer, Index 27 (96% over 25bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC1771.0895660203139428No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA1741.0710987996306556No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG1540.9479839950754079No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT1530.9418282548476453RNA PCR Primer, Index 27 (95% over 23bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG1100.6771314250538628No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG900.554016620498615RNA PCR Primer, Index 27 (100% over 24bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG830.5109264389042782No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA770.47399199753770394No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG760.46783625730994155No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCT560.3447214527546938No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG470.28931979070483227No Hit
GACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTAT330.20313942751615882No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC300.18467220683287164No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACA260.1600492459218221No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT250.1538935056940597RNA PCR Primer, Index 27 (96% over 28bp)
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC240.1477377654662973No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG230.14158202523853494No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT220.13542628501077256No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTGT210.12927054478301017RNA PCR Primer, Index 27 (95% over 23bp)
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA190.11695906432748539No Hit
CTAGTTTCCTCTGGCTTCCTGTCTCTTATACACATCTCCGAGCCCACGAG180.110803324099723No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT180.110803324099723No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC170.1046475838719606No Hit
CCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCG170.1046475838719606No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)