FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002295146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394685
Sequences flagged as poor quality0
Sequence length76
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG160814.074388436347974No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG152763.870428316252201No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA107002.7110227143164805No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG72881.8465358450409821No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT69221.7538036662148295No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCT55851.415052510229677No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTT53031.3436031265439528No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT49201.2465637153679516No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT40201.0185337674347898No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA39471.0000380049913222No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC38170.9671003458454209No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACAT36200.9171871239089401No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG35380.8964110619861407No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT33890.858659437272762No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC32790.8307891103031531No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT31350.7943043186338473No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT30890.7826494546283745No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT29880.7570594271380975No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACAT26230.6645806149207596No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC26180.6633137818766865No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC23960.6070663947198399No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG23950.6068130281110253No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC22530.5708349696593485No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC22290.5647541710477976No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC21670.549045441301291No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC21040.5330833449459697No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC19500.4940648871885174No Hit
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA18820.47683595778912297No Hit
TCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC18780.4758224913538645No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTT17960.45504642943106527No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCT17570.4451651316872949No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT16450.416788071500057No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC16330.4137476721942815No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTC15690.39753220923014554No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT15350.3889177445304483No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCT14410.36510128330187364RNA PCR Primer, Index 1 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT14170.35902048469032266TruSeq Adapter, Index 1 (95% over 22bp)
GATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACA14070.3564868186021764No Hit
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA13500.34204492189974284No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG12830.325069359109163No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACAT12800.32430925928271914No Hit
GATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACA12670.321015493368129No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA12650.3205087601504998No Hit
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACAT11490.2911182335280033No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACA11360.28782446761341324No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA11310.2865576345693401No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTA11160.2827571354371207No Hit
TCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC10930.2769297034343844No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG10890.2759162369991259No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC10870.27540950378149665No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTG10780.27312920430216503No Hit
CCCGATGAGCAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA10310.26122097368787767No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA10260.25995414064380457No Hit
CATCCATAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTC9960.2523531423793658No Hit
CGTCAGATGTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTT9770.24753917681188797No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC9560.24221847802678087No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATAC9540.2417117448091516No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC9510.24095164498270774No Hit
GTATAAGAGACAGCATCCGCAGCGTCATCAGCTGTCTCTTATACACATCT8940.22650974828027415No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC8890.22524291523620105No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCT8760.22194914932161092No Hit
CATCCATAGCGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAT8540.21637508392768917No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACAC8490.21510825088361607No Hit
ATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC8340.21130775175139666No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCT8300.2102942853161382No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCTGTCTC8210.20801398583680658No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCT8050.2039601200957726No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACA7960.20167982061644096No Hit
CATCCGCAGCGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAT7830.19838605470185083No Hit
CATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG7770.19686585504896312No Hit
CATCCATAGCGTCCTCAGCTGTCTCTCTGTCTCTTATACACATCTCCGAG7670.19433218896081686No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCATCAGCTGTCTCTT7580.19205188948148524No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTCTGTCTCTTATACACATCTCCGAG7410.18774465713163663No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCT7250.18369079139060265No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGT7060.17887682582312475No Hit
GCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGA6870.1740628602556469No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACA6790.1720359273851299No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACAT6780.1717825607763153No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGTCTCT6760.17127582755868603No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATAC6720.17026236112342752No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCT6670.16899552807935442No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACAC6490.1644349291206912No Hit
CATCCGCAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTC6410.1624079962501742No Hit
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGA6280.15911423033558408No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCT6270.15886086372676947No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCCTGTC6190.15683393085625244No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGT6110.15480699798573547No Hit
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACAT5980.15151323207114534No Hit
GTCCAGGGTGGACATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG5980.15151323207114534No Hit
GTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCT5970.15125986546233072No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCCTGTC5900.14948629920062836No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG5850.1482194661565552No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGTCTCT5830.14771273293892598No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCTGTCT5810.14720599972129672No Hit
CATCCATAGCGTCCTCAGCTGTCTCTGTCTCTTATACACATCTCCGAGCC5700.14441896702433588No Hit
GATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGA5680.14391223380670662No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCATCAGCTGTCTCTTATACACAT5590.141631934327375No Hit
CATCCGCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG5570.14112520110974575No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTGTCTCTTATACACATCTCCGAGCC5390.13656460215108251No Hit
CCCGATGAGCAGCACTACCTGTCTCCTGTCTCTTATACACATCTCCGAGC5330.13504440249819477No Hit
CCCGATGAGCAGCCACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC5290.13403093606293626No Hit
GCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGA5200.13175063658360464No Hit
GTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACAT5020.1271900376249414No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTG4990.12642993779849754RNA PCR Primer, Index 40 (96% over 26bp)
GACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCC4950.12541647136323905No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCCTGTCTCTTATACACATCTCC4910.12440300492798054No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACCTGTCTCTTATACACATCT4860.12313617188390742No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTA4860.12313617188390742No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTA4820.12212270544864892No Hit
CCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACA4770.1208558724045758No Hit
GCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC4740.12009577257813192No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTG4740.12009577257813192No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACCTGTCTCTTATACACAT4470.11325487414013707No Hit
GTGTATAAGAGACAGCTGAGGACGCTATGGATGCTGTCTCTTATACACAT4450.11274814092250782No Hit
CTAGTTTCCTCTGGCTTCCTGTCTCTTATACACATCTCCGAGCCCACGAG4430.11224140770487857No Hit
GATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACA4410.11173467448724933No Hit
GAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCT4360.11046784144317622No Hit
CATCCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGA4300.10894764179028846No Hit
CATCCATCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGA4300.10894764179028846No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTTTGCCGT4260.10793417535502996No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT4240.10742744213740071No Hit
GTGTATAAGAGACAGCTGAGGACGCTGCGGATGCTGTCTCTTATACACAT4210.10666734231095684No Hit
CCCGATGAGCAGCACTACCTCTGTCTCTTATACACATCTCCGAGCCCACG4090.10362694300518134No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCTGTCTCTTATA4040.10236010996110823No Hit
CATCCGCAGCGTCCTCATCCTGTCTCTTATACACATCTCCGAGCCCACGA4010.10160001013466435No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTC3970.10058654369940584No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)