FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002295149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392248
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG73791.8812078073055822No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCT41311.0531602455589322No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT31480.8025534865697213No Hit
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTGACGCTGCCGACG20160.5139605555668862No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTGACGCTGCCGACG18700.4767392058085701No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATC17980.4583834716811813No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTGACGCTGCCGACGAAGAG17960.45787359017764273No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTGACGC17640.44971548612102547No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATC14590.3719585568313924No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGAAGAG14350.3658399787889295No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGC14030.3576818747323122No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCCG13790.3515632966898493No Hit
TATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTG11220.28604352348514206No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTG10900.2778854194285248No Hit
GACAGTCGCTACGGGTCTGCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACC10510.2679427301095225No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCCTGTCTCTTATACACATCTGACGCTGCCG10490.267432848605984No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCG10380.2646285003365218No Hit
GAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTGACGCTGCCGA10270.2618241520670596No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTGACGCTGCCGACGAAGA10240.2610593298117518No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGCTGTCTCTTATACACATCTGACGCT9890.25213640349982663No Hit
GAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCG9840.25086169974098016No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAG9620.24525300320205587No Hit
GTATCAACGCAGAGTACATGGGGGTGTGGGTCTTGTCGCAGCTCTTGGGCTCGCTTCCGCTCTGGAAGTACAGGT9370.2388794844078236No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCCTGTCTCTTATACACATCTGACGCTGCCGA9250.23582019538659216No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTGTCTCTTATACACATCTGACGCTGCCGACG8920.22740715057820562No Hit
ACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTGACGCT8860.22587750606758988No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACCTGTCTCTTATACACATCTGACGCTGCCGACGAAG8620.21975892802512695No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCTGTCTCTTATACACATCTGACGCTGCCGA8470.21593481674858764No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCCGACGAAGA8430.2149150537415105No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTCTGTCTCTTATACACATCTGACGCT8400.21415023148620263No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGCTGTCTCTTATACACATCTGACGCTGCCGACGAAGAGGA8220.2095612979543554No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGCTGTCTCTTATACACATC8020.20446248291896962No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCGTAGCGACTGTCC7960.20293283840835388No Hit
CCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACG7850.20012849013889172No Hit
CCCCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGA7760.19783402337296813No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTGACG7340.187126511798658No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCTGTCTCTTATACA7270.18534192653627296No Hit
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTGACGCTGCCGACGAAGAG7210.18381228202565722No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCCTGTCTCTTATACACATCTGACGCTGCCGAC7190.18330240052211866No Hit
ATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTGA6850.17463441496196286No Hit
GTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGAAG6800.1733597112031164No Hit
CACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCT6760.17233994819603923No Hit
AGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGA6710.1710652444371928No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCC6550.16698619240888418No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCGTAGCGACTGTC6510.16596642940180703No Hit
CTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGAAGAGGA6440.164181844139422No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTGACGCTGCCGACGAAGAG6280.1601027921111134No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGCTGTCTCTTATACACATCTGACGCTGC6110.15576879933103546No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACG5960.15194468805449615No Hit
TATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTGACGCTGCCGACGAAGAGG5960.15194468805449615No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCC5940.15143480655095756No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATCTGTCTCTTATACACATCTGA5930.15117986579918827No Hit
GGTCTGCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACA5890.15016010279211112No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCGTAGCGACTGTCT5870.14965022128857253No Hit
CAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATC5720.14582611001203322No Hit
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCGTAGCGACTGTCCTGTCTCTTATACA5650.14404152474964818No Hit
TATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTGACGCT5540.141237176480186No Hit
GCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGAC5480.13970753196957025No Hit
GGTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCA5440.1386877689624931No Hit
GACAGTCGCTACGGGTCTGCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACA5210.13282413167179946No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTGACGCTGCCGACGAAGAGG5190.1323142501682609No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGCTGTCTCTTATACACATCTGACGC5110.1302747241541066No Hit
GTACATGGGGGTGTGGGTCTTGTCGCAGCTCTTGGGCTCGCTTCCGCTCTGGAAGTACAGGTTCTCATTCACAGT5040.12849013889172156No Hit
CCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATA5010.1277253166364137No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGCTGTCTCTTATACAC5010.1277253166364137No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCCTGTCTCTTATACACATCTGACGCTGCC4960.12645061287756726No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTGACGCT4950.12619567212579796No Hit
GTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCG4920.12543084987049008No Hit
ATGCTGGAGGTCGACCAGATGTCCGAAAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4840.1233913238563358No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACCTGTCTCTTATACACATCTGACGCTGCCGACGAA4770.12160673859395077No Hit
GGTATCAACGCAGAGTACATGGGGGTGTGGGTCTTGTCGCAGCTCTTGGGCTCGCTTCCGCTCTGGAAGTACAGG4680.11931227182802717No Hit
GCTGGAGGTCGACCAGATGTCCGAAAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4660.11880239032448858No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGCTGTCTCTTATACACAT4370.11140910852317921No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTGTCTCTTATACACATCTGACGCTGCCGAC4210.1073300564948706No Hit
GTATCAACGCAGAGTACATGGGGCTGTCTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTC4200.10707511574310132No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGCTGTCTCTTATACACATCTGACGCTGCCGACGAAGAGG4110.1047806489771777No Hit
CACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGC4100.10452570822540841No Hit
CAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGC4000.10197630070771552No Hit
ACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTGACGCTGCCGA3930.10019171544533051No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)