FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002295156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656138
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG248913.7935617202478746Illumina PCR Primer Index 10 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG90781.3835504116512074Illumina PCR Primer Index 10 (96% over 29bp)
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT79781.2159027521649408No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCT63070.961230716708985No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG52380.7983076730809677Illumina PCR Primer Index 10 (96% over 29bp)
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG46620.7105212622954317No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGG41030.6253257698837744No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTCCGAGCCCACGAG40800.6218204097308798No Hit
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG39110.5960636329552624No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATC35260.5373869521350692No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC32490.4951702233371639No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTCCGAG32390.4936461537054705No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGG29560.4505149831285492No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATC28830.43938927481718787No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTCCGAGCCCAC25330.3860468377079212No Hit
CACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGC22770.3470306551365719No Hit
CCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGC21890.33361884237767053No Hit
CCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAG21860.33316162148816253No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTC21220.32340757584532526No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG21190.32295035495581725No Hit
GTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC21040.3206642505082772No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCCTGTCTCTTATACACATCTCCGAGCCCACG20730.3159396346500279No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA20340.3099957630864239No Hit
GAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC19360.2950598806958292No Hit
TATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTC19270.2936882180273052No Hit
GAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACG18740.28561064897933053No Hit
CAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATC17960.27372290585212256No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG16660.25391000064010927No Hit
GCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA16650.25375759367693995No Hit
ACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTCCGAGC16320.24872816389235192No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTCTGTCTCTTATACACATCTCCGAGC16130.24583243159213458No Hit
AGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACG16010.24400354803410257No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGG15800.24080300180754657No Hit
GGTCTGCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACA15330.23363987453858792No Hit
CCCCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGA15100.23013451438569327No Hit
CCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG14820.22586711941695192No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTCCGA14370.21900880607433193No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC14320.21824677125848527No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA14160.2158082598477759No Hit
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGG14010.21352215540023592No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGC13950.2126077136212199No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCCTGTCTCTTATACACATCTCCGAGCCCAC13930.21230289969488125No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTCCGAGCCCA13870.21138845791586527No Hit
CTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAG13610.20742587687346262No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTGTCTCTTATACACATCTCCGAGCCCACGAG13470.20529217938909192No Hit
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG13170.2007199704940119Illumina PCR Primer Index 10 (96% over 29bp)
GACAGTCGCTACGGGTCTGCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACC13090.19950071478865727No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCTGTCTCTTATACA12920.1969097964147786No Hit
GTATCAACGCAGAGTACATGGGGGTGTGGGTCTTGTCGCAGCTCTTGGGCTCGCTTCCGCTCTGGAAGTACAGGT12640.19264240144603728No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGCTGTCTCTTATACACATC12250.18669852988243327No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGCTGTCTCTTATACACATCTCCGAGC12030.18334557669270796No Hit
ATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTCC12010.18304076276636927No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAG11830.18029743742932128No Hit
CAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCC11670.17785892601861192No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATCTGTCTCTTATACACATCTCC11640.17740170512910394No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGA11340.17282949623402394No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG10910.1662759968177426Illumina PCR Primer Index 10 (96% over 29bp)
GTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGC10840.16520914807555728No Hit
TATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTCCGAGC10590.16139897399632394No Hit
CCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATA10500.16002731132779996No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA10470.15957009043829196No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCTGTCTCTTATACACATCTCCGAGCCCACG10340.15758879991709063No Hit
CCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACAC10320.15728398599075194No Hit
CCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG9980.15210214924299462No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAG9930.15134011442714795No Hit
CACAAAATCTCACCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTG9910.15103530050080927No Hit
CCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACGA9360.14265291752649595No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACC9230.14067162700529462No Hit
ATGCTGGAGGTCGACCAGATGTCCGAAAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9080.13838552255775463No Hit
CAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACAT8900.1356421972207066No Hit
GTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC8850.13488016240485998No Hit
GGTCTGCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTCCGAGCCC8780.13381331366267463No Hit
TATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA8530.13000313958344129No Hit
CACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG8490.12939351173076394No Hit
GAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACC8440.1286314769149173No Hit
CTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC8310.12665018639371597No Hit
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCTGTCTCTTATACACATCTCCGAGCCC8190.12482130283568396No Hit
ACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACC8090.12329723320399061No Hit
GCTGGAGGTCGACCAGATGTCCGAAAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7990.12177316357229728No Hit
TCTCACCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTA7750.11811539645623328No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGCTGTCTCTTATACACAT7640.11643891986137063No Hit
GAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA7630.11628651289820129No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG7600.11582929200869328No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC7560.11521966415601596No Hit
GGTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCA7490.11415281541383063No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGCTGTCTCTTATACACATCTCCGAGCCC7380.11247633881896796No Hit
CTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA7320.11156189703995197No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC7300.1112570831136133No Hit
CTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA7170.10927579259241196Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCGTAGCGACTGTCC6890.10500839762367063No Hit
GCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACG6870.10470358369733196No Hit
CAATTGACGTTACCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAG6870.10470358369733196No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTCTGTCTCTTATACACATCTCCGAGCCCACG6830.10409395584465463No Hit
GGTATCAACGCAGAGTACATGGGGGTGTGGGTCTTGTCGCAGCTCTTGGGCTCGCTTCCGCTCTGGAAGTACAGG6750.10287470013929996No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGCTGTCTCTTATACACATCTCCGAG6710.10226507228662263No Hit
ATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTCCGAGCCCACGA6710.10226507228662263No Hit
AGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCAC6700.10211266532345328No Hit
CCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACG6660.10150303747077596No Hit
GTATCAACGCAGAGTACATGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG6660.10150303747077596No Hit
GGTCTGCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATAC6630.10104581658126796No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)