FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002295174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366200
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT36621.0No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCT28140.7684325505188422No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTCCGAGCCCACGAG20010.5464227198252322No Hit
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG19110.5218459858001092No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATC16990.4639541234298198No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATC14230.38858547241944297No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG13830.3776624795193883No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG13820.377389404696887Illumina PCR Primer Index 7 (96% over 27bp)
GACAGTCGCTACGGGTCTGCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACC11890.3246859639541234No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTC10130.2766247951938831No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTCCGAG9720.2654287274713272No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCCTGTCTCTTATACACATCTCCGAGCCCACG9710.2651556526488258No Hit
TATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTC8960.24467504096122336No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTCTGTCTCTTATACACATCTCCGAGC8860.2419442927362097No Hit
GAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACG8810.2405789186237029No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG8560.23375204806116875No Hit
GCTGGAGGTCGACCAGATGTCCGAAAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8530.23293282359366468No Hit
ACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTCCGAGC8460.23102129983615513No Hit
ATGCTGGAGGTCGACCAGATGTCCGAAAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8120.2217367558711087No Hit
GGTCTGCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACA7250.1979792463134899No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG7190.1963407973784817No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA6860.18732932823593665No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC6650.181594756963408No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG6600.18022938285090115No Hit
GAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC6260.17094483888585474No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCTGTCTCTTATACA6180.1687602403058438No Hit
ATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTCC5930.16193336974330966No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATCTGTCTCTTATACACATCTCC5880.16056799563080285No Hit
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG5570.15210267613326053No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTC5490.1499180775532496No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAG5430.1482796286182414No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCGTAGCGACTGTC5400.1474604041507373No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCGTAGCGACTGTCC5380.14691425450573456No Hit
GTATCAACGCAGAGTACATGGGGGTGTGGGTCTTGTCGCAGCTCTTGGGCTCGCTTCCGCTCTGGAAGTACAGGT5270.14391043145821955No Hit
CCCCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGA5160.14090660841070454No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCCTGTCTCTTATACACATCTCCGAGCCCAC5000.1365374112506827No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTCCGAGCCCAC4990.1362643364281813No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG4950.13517203713817585Illumina PCR Primer Index 7 (96% over 27bp)
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCGTAGCGACTGTCT4920.13435281267067176No Hit
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCGTAGCGACTGTCCTGTCTCTTATACA4860.1327143637356636No Hit
GGTCTGCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTCCGAGCCC4800.13107591480065536No Hit
GACAGTCGCTACGGGTCTGCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACA4690.12807209175314035No Hit
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCTGTCTCTTATACACATCTCCGAGCCC4680.127799016930639No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTCCGA4670.12752594210813764No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTC4650.1269797924631349No Hit
GCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA4570.12479519388312398No Hit
CCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAG4460.12179137083560897No Hit
GTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT4340.11851447296559257No Hit
CACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGC3870.1056799563080284No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA3850.10513380666302566No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGC3710.10131075914800655No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTGTCTCTTATACACATCTCCGAGCCCACGAG3710.10131075914800655No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT3700.10103768432550518No Hit
AGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACG3680.10049153468050245No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)