FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCT45.555555555555555Illumina PCR Primer Index 10 (95% over 22bp)
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT45.555555555555555No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTT45.555555555555555No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTT34.166666666666666No Hit
CAACCAAACGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTT22.7777777777777777No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCAACTCGTATGCCGTCTTCT11.3888888888888888Illumina PCR Primer Index 7 (95% over 21bp)
CAAGCAATCAAGTCCAGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCT11.3888888888888888Illumina PCR Primer Index 10 (95% over 22bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATTTCCGAGCCCACGAGATATCATCTAATCTCGTATGCCGTCTTCG11.3888888888888888RNA PCR Primer, Index 29 (95% over 24bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCAGCTCGTATGCCGTCTT11.3888888888888888No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCTAGCCCACGAGACATCATCTCATCTCGTATGCCTTCTT11.3888888888888888No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGATACATCGTCTCATCTCGTATGCCGTCTTCT11.3888888888888888Illumina PCR Primer Index 10 (95% over 22bp)
CTTCTTGTTTTCGGGACATGCTTCAGCGCCTAGCGCTCCTTGGTCACTGTCGCTCTGTCCACTCTTAACAGCCCC11.3888888888888888No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCTCACATG11.3888888888888888No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGCCATCATCTCATCTCTTATGCCGTCTT11.3888888888888888No Hit
CAAGCAATCTAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTGCT11.3888888888888888Illumina PCR Primer Index 7 (95% over 21bp)
ACATCATCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCACTAAGCTGGAGGACCGCATTGACTACCTGA11.3888888888888888No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCCATTGGGATCATG11.3888888888888888No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCTAGCCCACCAGACATCATCTCATCTCTTCTGCCCACTT11.3888888888888888No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACGTCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCT11.3888888888888888Illumina PCR Primer Index 10 (95% over 22bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGACCACGAGACATCATCTCATCTCGTATGCCGTCTT11.3888888888888888No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACTAGACATCATCTCATGCCGTATTCCGTCTT11.3888888888888888No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGGGGCACCAGCAGCGGGTAGCCGGCAA11.3888888888888888No Hit
CAACCAAAATAACAGCCTGTCTCTTATACACATCTCCGAGCCCACTAGACATCATCTCATCTCGTATGCCGTCTT11.3888888888888888No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCAGAGCCCACGAGACATCATCTCATCTNGTATGCCGTCTT11.3888888888888888No Hit
CAACCCAACGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATTCCGTCTT11.3888888888888888No Hit
GACACTTGTCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGGGGCACCAGCAGCGGGTAGCCGGCAA11.3888888888888888No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGTTCACTGTCGCTCTGTCCACTCTTAACGGCCCC11.3888888888888888No Hit
TCTCGTGCTCGCAGATGCCGCTCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGGGGCCCGCGCTTC11.3888888888888888No Hit
CAAGCAATCAAGGCCAGTCTCTTCTGCACATCTCCGAACCCACGAGACATCATCACAACGCGTAGGCCGTATTCT11.3888888888888888No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTTTCAGAATGGCCGGGATGCTGGTGGC11.3888888888888888No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACAGACATAATCTCAGCTCNTATGCCGTCTTC11.3888888888888888No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCACATCTCGTATGCCGTCTT11.3888888888888888TruSeq Adapter, Index 9 (95% over 22bp)
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAAGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG11.3888888888888888No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCCGTCGAGCACGGCCC11.3888888888888888No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGTAGGTCGNGAAGGGGCCCTG11.3888888888888888No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCAAGT11.3888888888888888No Hit
AAACTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGCCATCATCTCATCTCGTATGCCGTCTT11.3888888888888888No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACATCCTCTCATCTCGTATGCCGTCTTCT11.3888888888888888Illumina PCR Primer Index 7 (95% over 22bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACCTCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTT11.3888888888888888No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGA11.3888888888888888No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAAAAAACGGAGTGGAGCCATTCAGGTA11.3888888888888888No Hit
CAACCAAAAGAACATCCTGTCTCTTATACACATCTCCGCGCCCACGAGACATCATCTCATCTCGTATGCCGTCTT11.3888888888888888No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAGCGGGAGGACCGCATTGACTACCTGA11.3888888888888888No Hit
CAAGCAAAAGAACAGCCTGGCTCTTATACACATCTCCGAGACCACGAGACATCATCTCATCTCGTATGCCGTCTT11.3888888888888888No Hit
CTTCAGTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAGCTCACGAGACATCATCTCACCTCTTATGC11.3888888888888888No Hit
ACCCTCCCTGACCAATATGTCTCTTATACACATCTCCTAGCCCACGCGACATCATCTCGTATCGTATGCCGTCTT11.3888888888888888No Hit
AAACTCCCTGACCAATCTATCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTT11.3888888888888888No Hit
TCTCGTTCTCGCAGATGCCGCCCATCGGCTCCTGTGCGTCAGTTTGGGCGGTAGGCTGCGCGNGGCCCGCGCCCC11.3888888888888888No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTG11.3888888888888888No Hit
CAAGCAATCGAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCT11.3888888888888888Illumina PCR Primer Index 10 (95% over 22bp)
CAAGCAAAAGAACAGCCTGTCTCCTATACACATCTCCGAGCTCACGAGACATGATCTCATCTCGCATGCCGTCTT11.3888888888888888No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAT11.3888888888888888No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCACACGAGACATCATCTCATCTCGTATGCCGTCTT11.3888888888888888No Hit
GTTTCCAGGTCAAGTGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTTA11.3888888888888888No Hit
CAACCAAAAGCACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTT11.3888888888888888No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG11.3888888888888888No Hit
CAACCAAAAGAACCGCCTGTCTCTTATACACATCTCCGAGCCCACTAGACATCATCTCAGCTCGTATGCATTCTT11.3888888888888888No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAGCATCTCATCTCGTATGCCGTCTTCT11.3888888888888888Illumina PCR Primer Index 10 (95% over 22bp)
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGTCCTCCTTGGTCACTGTCGCTCTGTCCACTCTTAACGGCCCC11.3888888888888888No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACGCATCTCCGAGCCCACGAAACATGATCTCATATCGTATGCCGTCTT11.3888888888888888No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)