FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT5939.86486486486486No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCGCATTGACTACCTGA2214.864864864864865No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCAACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT85.405405405405405No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCCGTCCAGCACGGCCC42.7027027027027026No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCT21.3513513513513513Illumina PCR Primer Index 6 (95% over 22bp)
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTTCTTGGCCTTGTCGCGGCTCTT21.3513513513513513No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTNGTCGCGGCTCTT21.3513513513513513No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAAACCACTGCTGCAGAACTTGATAGCCACAC21.3513513513513513No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTTTCGCGGCTCTT21.3513513513513513No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCTAGCCCACGAGACCCACGTATAGCTCTTATGCCGTCTT10.6756756756756757No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCGCATTGACGACCTGA10.6756756756756757No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCTTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT10.6756756756756757No Hit
GGCTGTGGTGTGAGTNCGGGGGGCGGCCGTAGCGCACCATTTGCACCCGCAGCGCGCGGCCGTCCAGCACGGCCC10.6756756756756757No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACGAAAGCCACCACAGCAATAAGCTGGAGGACCGCATTGACTACCTGA10.6756756756756757No Hit
GACATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCAGCTCTT10.6756756756756757No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCGT10.6756756756756757No Hit
GTCATTGTCACTGGTCAGCTCTAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCGTTGTCGCGGATCTT10.6756756756756757No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTT10.6756756756756757No Hit
GTCAGTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCAACGTTGCGCTGCTTGGCCTTGTCGCGGCGCTT10.6756756756756757No Hit
GTCATTTTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT10.6756756756756757No Hit
GTCATTGTCACTGGTCAGCTCCAACACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT10.6756756756756757No Hit
GTCATTGTCACTGGCCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT10.6756756756756757No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCGCATTGACTACATGA10.6756756756756757No Hit
GTCATTGTCACTGGTAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTTG10.6756756756756757No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCTTATGCCGTCTTCT10.6756756756756757TruSeq Adapter, Index 6 (95% over 21bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGATACCCACGTATATCTCGTATGCCGTCTTCT10.6756756756756757RNA PCR Primer, Index 37 (95% over 23bp)
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAGCTCGTATGCCGTCTT10.6756756756756757No Hit
ACATCATCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCGCATTGACTACCTGA10.6756756756756757No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCAACGTTGCGATGCTTGGCCTTGTCGCGGCTCTT10.6756756756756757No Hit
GTCATTTTCACTGGTCCGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCTGCTCTT10.6756756756756757No Hit
ACATCAACCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCACTAAGCTGGAGGACCGCATTGACTACCTGA10.6756756756756757No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCTCGGCTCTT10.6756756756756757No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTTCGCTGCTTGTCCTTGTCGCGGCTCTT10.6756756756756757No Hit
GTCATTGTCACTGCTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT10.6756756756756757No Hit
ACATCAGCCTCTATATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGATGAAGGACCGCATTGACTACCTGA10.6756756756756757No Hit
GTCATTTTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGTCCTTTTCGCGGCTCTT10.6756756756756757No Hit
GTCATTGCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGTCCTTNTCGCGGCTCTTT10.6756756756756757No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAACCCACGAGACCCACGTATATCTCGTATGCCGTCTTCT10.6756756756756757RNA PCR Primer, Index 37 (95% over 23bp)
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCGACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT10.6756756756756757No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGTCGTCAAGCACGGCCC10.6756756756756757No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAACGTATATCTCGTATGCCGTCTT10.6756756756756757RNA PCR Primer, Index 37 (95% over 21bp)
GTCATTGTCACTGGTCCGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT10.6756756756756757No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCGCTT10.6756756756756757No Hit
GTCATTGTCACTGGTCATCTCCAGTACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT10.6756756756756757No Hit
GTCATTGTCACTGATCAGCTCCAGCACATTCTGCTGCGTCTCCACGTTGCGCTGCTTTGCCTTGTCGCGGCTCTT10.6756756756756757No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATACCTCGTATGCCGTCTT10.6756756756756757No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCGCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT10.6756756756756757No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCGACGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT10.6756756756756757No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGCGCCCACGAGACCCACGTATATCTNGTATGCCTACTT10.6756756756756757No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGTAGGACCGCATTGACTACCTGA10.6756756756756757No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCTCATTGACTACCTGA10.6756756756756757No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCGTTGTCGCGGCACTT10.6756756756756757No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTNGTATGCCGAATT10.6756756756756757No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACTTTGCGCTGCTTGGCCTTGTCGCGGCTCTT10.6756756756756757No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)