FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98392
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG62016.302341653792991No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG61446.244410114643467No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC57205.813480770794373No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT53235.409992682331897No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG52365.321570859419465No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT50835.1660704122286365No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT50245.106106187494918No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG48644.943491340759412No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC47894.867265631352143No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC45054.578624278396617No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA41654.233067729083665No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG36353.694406049272299No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT29482.996178551101716No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA29152.9626392389625176No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG27372.7817302219692657No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAG26582.7014391413936094No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG25702.6120009756890803No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTT24792.5195137816082607No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT23882.427026587527441No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC21142.148548662492886No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG19641.9960972436783477No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC12801.3009187738840557No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATA7880.8008781201723717No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAACCAC7620.7744532075778519No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATT7020.7134726400520368No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAAACCA6190.6291161883079925No Hit
CCTGACTCACGTTTCCTTATTGCTGGGATGTTCACAGAGCCAACGTCCTG5790.5884624766241158No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT5000.5081713960484592No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCT4330.4400764289779657No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGGCACCGGGTCATTCTTTT3480.3536872916497276No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGACACCGGGTCATTCTTTT2380.2418895845190666No Hit
CTGTTTTCATCCCACAGGTTGTTATTTTGTACTTTGAGCCAAGCACATTT2020.20530124400357752No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)