FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002295241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590463
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG45490.7704123713086171RNA PCR Primer, Index 39 (96% over 30bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGC25070.4245820652606514No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG20410.345660947426003RNA PCR Primer, Index 39 (96% over 30bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG16130.27317545722593967RNA PCR Primer, Index 39 (96% over 30bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC14500.24557000184600897RNA PCR Primer, Index 39 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG13820.23405361555254098RNA PCR Primer, Index 39 (96% over 30bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC13090.22169043614925915RNA PCR Primer, Index 39 (95% over 21bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG12990.21999684992963148RNA PCR Primer, Index 39 (96% over 30bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC12920.21881133957589216RNA PCR Primer, Index 39 (95% over 21bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGC9110.15428570460807875No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC8450.14310803555853627RNA PCR Primer, Index 39 (95% over 21bp)
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG8350.14141444933890862Illumina PCR Primer Index 11 (96% over 29bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG6500.11008310427579712No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG6160.10432491112906313No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)