FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002295281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342315
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGC24550.7171757007434673No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG16830.49165242539766TruSeq Adapter, Index 11 (96% over 30bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGC8050.23516351898105547No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG6950.20302937353022799No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG6430.18783868658983685RNA PCR Primer, Index 11 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG6430.18783868658983685RNA PCR Primer, Index 11 (96% over 30bp)
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAG6090.17790631435958107No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCT5250.15336751237894922No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG5160.15073835502388153TruSeq Adapter, Index 11 (96% over 30bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC3790.11071673750785095TruSeq Adapter, Index 11 (95% over 21bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAG3770.11013248031783592No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCT3580.10458203701269297No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC3500.1022450082526328TruSeq Adapter, Index 11 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)