FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002295292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076813
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGC282802.6262684421529086No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTC124171.153125008706247RNA PCR Primer, Index 47 (95% over 21bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTC72280.6712400388925468RNA PCR Primer, Index 47 (95% over 21bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGA67710.6287999866272045No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAA66480.6173773905032722No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTC65410.6074406605418025RNA PCR Primer, Index 47 (95% over 21bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGC56630.5259037548766592No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG49650.4610828435392218RNA PCR Primer, Index 47 (96% over 30bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTC45250.42022152407149616RNA PCR Primer, Index 47 (95% over 21bp)
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTG39040.3625513436409107No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGA32180.29884483192532035No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG28940.26875604213544974No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTG21620.20077766520277895No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGA19150.17783960631976026No Hit
CCAGAGGGGAAAAATATGACAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA17590.16335241123574845Clontech Universal Primer Mix Long (96% over 26bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG17060.1584304795725906RNA PCR Primer, Index 47 (96% over 30bp)
CCAGAGGGGAAAAATATGACAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT16170.15016534904389156Clontech Universal Primer Mix Long (96% over 26bp)
CCAGAGGGGAAAAATATGACAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG15440.14338608467765526Clontech Universal Primer Mix Long (96% over 26bp)
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGAT15200.14115728543396114No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACG14860.1379998198387278No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC13740.12759875670148857No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATG12840.1192407595376356No Hit
ACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG12780.11868355972671207RNA PCR Primer, Index 47 (96% over 30bp)
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC12630.11729056019940325No Hit
CTCTTTTCCCACTTCCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG12490.11599042730724833No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTC11780.10939689621131989RNA PCR Primer, Index 47 (95% over 21bp)
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAG11430.10614656398093264No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)