FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002295293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3269613
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGC34196910.459005392992992No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC1615514.940982311973925No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC1052873.220167035058889No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC989213.0254650932694482No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGC924142.8264507145035207No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCT801122.450198234469951No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCA799122.44408130258841No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC709402.1696757383824936No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC166180.5082558700372185No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG149390.45690422689168414Illumina PCR Primer Index 7 (96% over 27bp)
CCCGATGAGCAGCCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTT137660.4210284214064478No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAT115080.3519682604638531No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATC113780.3479922547408516No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCC106640.32615480792375123No Hit
GTCCAGGGTGGACATGGGCACACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGC99880.3054795781641436No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG96990.29664061159531724No Hit
CTAGTTTCCTCTGGCTTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC91220.2789932631170723No Hit
GGTCGGTAGTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGC86260.26382327205085127Illumina PCR Primer Index 7 (95% over 24bp)
CCAGAGGGGAAAAATATGACAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA76910.23522661550464838Clontech Universal Primer Mix Long (96% over 26bp)
TCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC76120.23281042741143984No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAT69550.21271630618057855No Hit
CCAGAGGGGAAAAATATGACAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT69470.21247162890531696Clontech Universal Primer Mix Long (96% over 26bp)
CATCCGCAGCGTCCTCATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGT69250.21179876639834747No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCC65220.19947314865704288No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG63630.19461018781121803TruSeq Adapter, Index 18 (96% over 29bp)
GCTCTCAGCTCCGTAGTGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCG63220.19335621677550216No Hit
CCAGAGGGGAAAAATATGACAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG61230.18726986955336916Clontech Universal Primer Mix Long (96% over 26bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCAGCTCGTATGC59800.18289626325806754No Hit
GCTCTCAGCTCCGTAAAGGTGGAGCTCATCTTCATTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAT59700.1825904166639905No Hit
CCCGATGAGCAGCACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC58940.18026598254900503No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATG56620.1731703415664178No Hit
GTTTCCAGGTCAAGGGTAACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCA56510.17283391031293305No Hit
GCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC55790.1706318148355784No Hit
CCCGATGAGCAGCCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGT54490.16665580911257694No Hit
CATCCGCAGCGTCCTCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC54320.16613586990264598No Hit
CATCCATAGCGTCCTCATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGT53880.16479014488870702No Hit
GGTCATTGTCACTGGTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGT49760.15218926521273313No Hit
GTTTCCAGGTCAAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATC44780.13695810482769674No Hit
GTCCAGGGTGGGCATGGGCACACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGC44640.1365299195959889No Hit
GTTTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTAT43950.13441957809685734No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACC42830.13099409624319452No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATATCGTATGC42700.13059649567089437No Hit
CATCCATAGCGTCCTCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC41590.12720159847663928No Hit
AAAGTTGAAATGTCAGGGTCATGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCT41150.12585587346270033No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCAT41090.1256723655062541No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC40820.12484657970224611No Hit
AAAGTTGAAATGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTC39350.12035063476931367No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC39320.12025888079109055No Hit
CCAGAGGGGAAAAATATGACAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT38020.1162828750680891Clontech Universal Primer Mix Long (96% over 26bp)
GTTTCCACGTCAGAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCG36820.11261271593916465No Hit
CCAGAGGGGAAAAATATGACAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA35250.1078109244121552Clontech Universal Primer Mix Long (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG34220.10466070449316173TruSeq Adapter, Index 18 (96% over 29bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTC34130.1043854425584924No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)