FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002295295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4479288
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC77487017.29895465529343No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC3954338.828032490878014RNA PCR Primer, Index 32 (95% over 24bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC3706588.27493119442197RNA PCR Primer, Index 32 (95% over 24bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC3289537.343868043314027No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC3130016.987740015823943RNA PCR Primer, Index 32 (95% over 24bp)
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTC2182154.871644779259561No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC2155054.811144092543279RNA PCR Primer, Index 32 (95% over 24bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAA1738863.8820008894270694No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC466321.0410583110530067RNA PCR Primer, Index 32 (95% over 24bp)
GGTCGGTAGTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGC339230.7573301828326288RNA PCR Primer, Index 32 (96% over 30bp)
CCCGATGAGCAGCCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTT296060.6609532586428915RNA PCR Primer, Index 32 (96% over 26bp)
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATC287300.6413965790991782No Hit
CTAGTTTCCTCTGGCTTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC248990.5558695935604051RNA PCR Primer, Index 32 (95% over 24bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGC248860.5555793688639801No Hit
GTCCAGGGTGGACATGGGCACACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC206190.46031869350664656No Hit
CTGCTTCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTG177820.39698273475605944RNA PCR Primer, Index 32 (96% over 33bp)
CATCCGCAGCGTCCTCATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGT167350.3736084842055255RNA PCR Primer, Index 32 (95% over 23bp)
TCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC159900.3569763766027101RNA PCR Primer, Index 32 (95% over 24bp)
AAAGTTGAAATGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTC158990.3549448037277353No Hit
GCTCTCAGCTCCGTAGTGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCG158850.35463225405466225RNA PCR Primer, Index 32 (95% over 22bp)
GCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC157220.35099328286102616RNA PCR Primer, Index 32 (95% over 24bp)
CATCCATAGCGTCCTCATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGT155160.34639433767152283RNA PCR Primer, Index 32 (95% over 23bp)
GTCCAGGGTGGGCATGGGCACACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC142590.31833184202489323No Hit
CCCGATGAGCAGCACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC142210.3174834929122664RNA PCR Primer, Index 32 (95% over 24bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCCGTC141510.3159207445469012No Hit
GCTCTCAGCTCCGTAAAGGTGGAGCTCATCTTCATTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCA134390.30002536117347217No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCCGTC134080.2993332868973819No Hit
GGTCATTGTCACTGGTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGT124990.27903988312428224RNA PCR Primer, Index 32 (95% over 23bp)
AAAGTTGAAATGTCAGGGTCATGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTC113770.25399125932514277No Hit
GTTTCCAGGTCAAGGGTAACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAA111700.24936998915899133No Hit
GTTTCCACGTCAGAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCG110270.2461775174983167RNA PCR Primer, Index 32 (95% over 22bp)
CATCCGCAGCGTCCTCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC110060.24570869298870712RNA PCR Primer, Index 32 (95% over 24bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCCGTC107450.23988187408355968No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGC103300.23061700877460883No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCCGTC103240.23048305891472035No Hit
CTTCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC100340.22400881568677877RNA PCR Primer, Index 32 (95% over 24bp)
CCCGATGAGCAGCCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGT100090.22345069127057693RNA PCR Primer, Index 32 (95% over 23bp)
CATCCATAGCGTCCTCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC99420.22195491783515595RNA PCR Primer, Index 32 (95% over 24bp)
GTTTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTAT99090.2212181936057695No Hit
GTTTCCAGGTCAAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATC94890.21184170341357825No Hit
AAAGTTGAAATATCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTC92960.20753298292049988No Hit
CAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTG88600.1977992931019394RNA PCR Primer, Index 32 (96% over 33bp)
CTTCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC84490.18862372769958083RNA PCR Primer, Index 32 (95% over 24bp)
GTTTCCAGGTCAGAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCG79190.17679149007610137RNA PCR Primer, Index 32 (95% over 22bp)
CTAGTTTCCTTTGGCTTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC74250.16576295161195262RNA PCR Primer, Index 32 (95% over 24bp)
CATCCGCAGCGTCATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC73380.16382067864357014RNA PCR Primer, Index 32 (95% over 24bp)
GTCCAGGGTGGAGCTCATCTTCATTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGT72240.16127563130568964No Hit
GGTCATTGTCACTGCTATTTCCTCTGGCTTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAAT63710.14223242622488216No Hit
GTTTCCAGGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAAT50430.11258485723623934No Hit
CATCCGCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTG48580.10845473655634555RNA PCR Primer, Index 32 (96% over 33bp)
GTCCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTAT47080.10510599005913439No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAAGCTCGTATGC46160.10305209220751155No Hit
GGCTGTGGTGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGC46090.10289581737097503RNA PCR Primer, Index 32 (96% over 30bp)
CATCCGTAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC45970.10262791765119814RNA PCR Primer, Index 32 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)