FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT805.17464424320828No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT422.716688227684347RNA PCR Primer, Index 27 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT251.6170763260025873RNA PCR Primer, Index 27 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC241.5523932729624839No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG181.1642949547218628RNA PCR Primer, Index 27 (100% over 24bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA120.7761966364812419No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTGT100.646830530401035RNA PCR Primer, Index 27 (95% over 23bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG100.646830530401035No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG100.646830530401035No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG90.5821474773609314No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG90.5821474773609314No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCT90.5821474773609314No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC70.45278137128072443No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT70.45278137128072443RNA PCR Primer, Index 27 (96% over 28bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG60.38809831824062097No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACAT60.38809831824062097No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC50.3234152652005175No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA50.3234152652005175No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT50.3234152652005175No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC40.258732212160414No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCT40.258732212160414No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC40.258732212160414No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCT40.258732212160414No Hit
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA40.258732212160414No Hit
GACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTAT30.19404915912031048No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACAT30.19404915912031048No Hit
TCCCTATACGCGATGGATCGAGGTCATGACTGGACACTGCATCGGAAGAC30.19404915912031048No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC30.19404915912031048No Hit
GTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTG30.19404915912031048No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC30.19404915912031048No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTTTGCCGT30.19404915912031048No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC30.19404915912031048No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG30.19404915912031048No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT30.19404915912031048No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCT30.19404915912031048No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCT30.19404915912031048No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTG30.19404915912031048No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC30.19404915912031048No Hit
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA20.129366106080207No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA20.129366106080207No Hit
GTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC20.129366106080207No Hit
GTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCT20.129366106080207No Hit
GTTTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG20.129366106080207No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACCTG20.129366106080207No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCT20.129366106080207No Hit
GCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGGAAGGAGACA20.129366106080207No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCTGTCTC20.129366106080207No Hit
AAAGTTGAAATGTCAGGGTCATGTCCTTCTTGATCTGTCTCTTATACACA20.129366106080207No Hit
GTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAAC20.129366106080207No Hit
GCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCCTATTGGGA20.129366106080207No Hit
GTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGG20.129366106080207No Hit
ATGTTATTCCTAATAGGGGTCCTTATCCTTATAATCAACCCAAACGTAAT20.129366106080207No Hit
GTACATGGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGC20.129366106080207No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA20.129366106080207No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCCTGTCTC20.129366106080207No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT20.129366106080207No Hit
GTCCAGGGTGGACATGGGCCTGTCTCTTATACACATCTCCGAGCCCACGA20.129366106080207No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGT20.129366106080207No Hit
GCCAAGCTCCCTACTGTGTCAGAAATGTGCGTTTTGGAACCAAGCTTGGA20.129366106080207No Hit
TCCTTCTTGATCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCT20.129366106080207No Hit
TCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGT20.129366106080207No Hit
GTTATAATCGACCCGGGCTGCGACAATCGGCCGTATGCGGCGGTTGTTCT20.129366106080207No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC20.129366106080207No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGGCGTCTT20.129366106080207No Hit
GTGTATAAGAGACAGATACACATCTCCGAGCCCACGAGACGGACTCCTAT20.129366106080207No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT20.129366106080207No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA20.129366106080207No Hit
CCACACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGT20.129366106080207No Hit
ACACGAGGATGTTCCTGTTGATACCGTCTGCGATAGGCTAGTTCATAAAC20.129366106080207No Hit
CCCGATGAGCAGCCACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC20.129366106080207No Hit
CATCCATAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACT20.129366106080207No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC20.129366106080207No Hit
TAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAG20.129366106080207No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT20.129366106080207No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATG20.129366106080207No Hit
ATTATCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTTGT20.129366106080207No Hit
GTCCAGGGTGGACATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG20.129366106080207No Hit
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACGG20.129366106080207No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGTCTCT20.129366106080207No Hit
ACCCTATGTTGTAGCCACAGCTGGAGCCTGAGTCCGCTGCACGGAGACTC20.129366106080207No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCCTGGAGATTGTCTCGTACG20.129366106080207No Hit
CAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCT20.129366106080207No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCT20.129366106080207No Hit
ACGCTAGTCTAGCCTAGTAAAACCACGATCAGCCGACGGTCTGGATGCCG20.129366106080207No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCT20.129366106080207No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATATCGTATGCCGTCTT20.129366106080207No Hit
ATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA20.129366106080207No Hit
GAGATGTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCT20.129366106080207No Hit
CTTCCTCCCTCATTTCCTAGGGGGAGATGGCCCGTGTCTGACCCCCCAGC20.129366106080207No Hit
TAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGCTC20.129366106080207No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACAC20.129366106080207No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCGT20.129366106080207RNA PCR Primer, Index 27 (95% over 21bp)
CAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT20.129366106080207No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTC20.129366106080207No Hit
CCGTCAGGCAGTCGCTGCGCGCGGCGGAGACGAAGGCGTCTTCCAGCTCC20.129366106080207No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGG20.129366106080207No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACA20.129366106080207No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACAT20.129366106080207No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT20.129366106080207No Hit
CGTCAGATGTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTT20.129366106080207No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC20.129366106080207No Hit
GGAAGGAGACATAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTT20.129366106080207No Hit
CGTCAGCCCTGTTCCGTTTTTGTGTGACGCTGGTCAGTACTTTTCCGTCT20.129366106080207No Hit
ATATACAGCTCCTTGGAATGGTCAAAGCACCCCCTGACCTTCACTGGCCT20.129366106080207No Hit
CGGTGACAAGGTTTCCCCCTAATCGAGACGCTGCAATAACACAGGGGCAT20.129366106080207No Hit
CTACTAAAAAATACAAAAAATTAGCTGGGCGCAGAGGCACGGGCCTGTAG20.129366106080207No Hit
AGTGTGGGAAAAGGGTGACAGAGATAACAGAATCAACTTTCTTGAATGTA20.129366106080207No Hit
ATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA20.129366106080207No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCAT20.129366106080207RNA PCR Primer, Index 27 (95% over 21bp)
ATTCAGCTCTCAGTGCCGTGGGACTTCCTTGTCTGATGTACTGTTGAGCA20.129366106080207No Hit
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACAT20.129366106080207No Hit
GATGTGTATAAGAGACAGCGTGCCCATGCCCACCCTGGACCTGTCTCTTA20.129366106080207No Hit
GAAGTGAACATAACCTGTAGCCACAACAACATTGCTACAAATGATTATAT20.129366106080207No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTT20.129366106080207No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)