FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8742
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACAT660.7549759780370624No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCCTGTCTCTTATACACATCTCCGA650.7435369480668039No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT570.6520247083047358No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACAT530.6062685884237017No Hit
AGTCTGGTGTTCCTGTTCATTCAAGAGACTGTCTCTTATACACATCTCCG530.6062685884237017No Hit
AGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACGACGACGATCACGCG510.5833905284831846No Hit
TCTCTTGAATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCG510.5833905284831846No Hit
CATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATC500.5719514985129261No Hit
GAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGA450.5147563486616334No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATC440.503317318691375No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACAT400.4575611988103409No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTC400.4575611988103409No Hit
GTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACAT380.43468313886982385No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC360.41180507892930684No Hit
AGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATC330.3774879890185312No Hit
GCCAAGAAAGCACGAGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACG310.3546099290780142No Hit
GAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCG310.3546099290780142No Hit
CCCTGGAATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACC300.34317089910775567No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATC290.33173186913749714No Hit
TTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCG290.33173186913749714No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCG280.3202928391672386No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTT280.3202928391672386No Hit
GTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGA270.3088538091969801No Hit
GCACGAGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACGACGACGATC270.3088538091969801No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCCTGTCTCTTATACACATCTCCG260.29741477922672155No Hit
CTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGAGGCACG260.29741477922672155No Hit
GAAAGCACGAGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACGACGAC260.29741477922672155No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTC250.2859757492564631No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATA250.2859757492564631No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTAT240.27453671928620454No Hit
CCTCCAGGTCGAATCTTCAAACGACGACGATCACGCGTCGCTCCAGTATT240.27453671928620454No Hit
TTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGA240.27453671928620454No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGT230.263097689315946No Hit
ATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGAGGC230.263097689315946No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGA220.2516586593456875No Hit
GATGAACCCTGGAATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGAT220.2516586593456875No Hit
GTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGA210.24021962937542896No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCT210.24021962937542896No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA210.24021962937542896No Hit
AAAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACA210.24021962937542896No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACAT210.24021962937542896No Hit
CTGGAATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTG210.24021962937542896No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTAT200.22878059940517045No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGACTGTCTCTTATACACATCT190.21734156943491192No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACAT180.20590253946465342No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTA180.20590253946465342No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATA180.20590253946465342No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACCTGTCTCTTATACACAT170.19446350949439486No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGT170.19446350949439486No Hit
TGGTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCC170.19446350949439486No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTTTTCTGTCTCTTATACACA160.18302447952413636No Hit
ACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGAGGCACGGCTGATGC160.18302447952413636No Hit
GCTGATGCTCGTGCTTTCTTGGCAATAAGTACCGTAGGATCTGATGAACC160.18302447952413636No Hit
GTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATA160.18302447952413636No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACATC160.18302447952413636No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTC160.18302447952413636No Hit
GTACCGTAGGATCTGATGAACCCTGGAATACTGGAGCGACGCGTGATCGT150.17158544955387783No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTG150.17158544955387783No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGACTGTCTCTTATACACAT150.17158544955387783No Hit
GTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCG150.17158544955387783No Hit
TCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCC150.17158544955387783No Hit
CCTGGAGGCACGGCTGATGCTCGTGCTTTCTTGGCAATAAGTACCGTAGG150.17158544955387783No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTC140.1601464195836193No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGCTGTCTCTTATACA140.1601464195836193No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTC140.1601464195836193No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTT140.1601464195836193No Hit
CTGATGAACCCTGGAATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAG140.1601464195836193No Hit
CTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATA140.1601464195836193No Hit
CATCAGCCGTGCCTCCAGGTCGAATCTTCAAACGACGACGATCACGCGTC140.1601464195836193No Hit
CGTGATCGTCGTCGTTTGAAGATTCGACCTGGAGGCACGGCTGATGCTCG130.14870738961336077No Hit
GGTCGAATCTTCAAACGACGACGATCACGCGTCGCTCCAGTATTCCAGGG130.14870738961336077No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACCTGTCTCTTATACA130.14870738961336077No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTC130.14870738961336077No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCG130.14870738961336077No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCT130.14870738961336077No Hit
GGTGTTCCTGTTCATTCAAGAGACTGTCTCTTATACACATCTCCGAGCCC130.14870738961336077No Hit
TCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCT130.14870738961336077No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACAT120.13726835964310227No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTC120.13726835964310227No Hit
GTTCATCTCTTGAATGAACAGGAACACCACTGTCTCTTATACACATCTCC120.13726835964310227No Hit
GTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTC120.13726835964310227No Hit
GTGTTCCTGTTCATTCAAGAGATGCTGTCTCTTATACACATCTCCGAGCC120.13726835964310227No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCT120.13726835964310227No Hit
AAAAAAAGTCTGGTGTTCCTGTTCATCTCCTGTCTCTTATACACATCTCC120.13726835964310227No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCT120.13726835964310227No Hit
AGGCACGGCTGATGCTCGTGCTTTCTTGGCAATAAGTACCGTAGGATCTG120.13726835964310227No Hit
TTCCTGTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACATC120.13726835964310227No Hit
TGCCAAGAAAGCACGAGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAAC110.12582932967284374No Hit
CCTGTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACATCTC110.12582932967284374No Hit
GGAATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGA110.12582932967284374No Hit
GATTCGACCTGGAGGCACGGCTGATGCTCGTGCTTTCTTGGCAATAAGTA110.12582932967284374No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTG100.11439029970258523No Hit
CCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACA100.11439029970258523No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACA100.11439029970258523No Hit
ATCGTCGTCGTTTGAAGATTCGACCTGGAGGCACGGCTGATGCTCGTGCT100.11439029970258523No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCT100.11439029970258523No Hit
TCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCA100.11439029970258523No Hit
GCTCCAGTATTCCAGGGTTCATCAGATCCTACGGTACTTATTGCCAAGAA100.11439029970258523No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGC100.11439029970258523No Hit
GCCGTGCCTCCAGGTCGAATCTTCAAACGACGACGATCACGCGTCGCTCC100.11439029970258523No Hit
TCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGC100.11439029970258523No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTC100.11439029970258523No Hit
TCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC100.11439029970258523No Hit
GTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGACTCTGTCTCTT100.11439029970258523No Hit
GCGTGATCGTCGTCGTTTGAAGATTCGACCTGGAGGCACGGCTGATGCTC100.11439029970258523No Hit
GCAATAAGTACCGTAGGATCTGATGAACCCTGGAATACTGGAGCGACGCG100.11439029970258523No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTA90.10295126973232671No Hit
AGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCG90.10295126973232671No Hit
AAAAAAAGTCTGGTGTTCCTGTTCATCCTGTCTCTTATACACATCTCCGA90.10295126973232671No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACCTGTCTCTTATACACA90.10295126973232671No Hit
AAGAAAGCACGAGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACGACG90.10295126973232671No Hit
GCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACGACGACGATCACGCGT90.10295126973232671No Hit
CTTCAAACGACGACGATCACGCGTCGCTCCAGTATTCCAGGGTTCATCAG90.10295126973232671No Hit
ATTCCAGGGTTCATCAGATCCTACGGTACTTATTGCCAAGAAAGCACGAG90.10295126973232671No Hit
AAAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTG90.10295126973232671No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGACTGTCTCTTATACACATCTC90.10295126973232671No Hit
CCAGTATTCCAGGGTTCATCAGATCCTACGGTACTTATTGCCAAGAAAGC90.10295126973232671No Hit
CATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCC90.10295126973232671No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGT90.10295126973232671No Hit
CAAGAAAGCACGAGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACGAC90.10295126973232671No Hit
ACCTGGAGGCACGGCTGATGCTCGTGCTTTCTTGGCAATAAGTACCGTAG90.10295126973232671No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTC90.10295126973232671No Hit
CTTATACACATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC90.10295126973232671No Hit
GGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGAGGCACGGC90.10295126973232671No Hit
AAGCACGAGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACGACGACGA90.10295126973232671No Hit
GGTGTTCCTGTTCATTCAAGAGATGCTGTCTCTTATACACATCTCCGAGC90.10295126973232671No Hit
GAATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGAG90.10295126973232671No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)