FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002295350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924176
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA27650.2991854365402261No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA26860.29063728121050536No Hit
GTGATAATCAGCCTCATCCTCAGACTGGAGCCCGCTGATGGCCAGGGAGG26140.2828465573656966No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT25180.2724589255726182No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT23750.25698568238084524No Hit
GATTATCACTGTGCAACATGGGATGCCACCTTGGATGGTTATGTCTTCGG19610.2121890202731947No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT18910.20461470542407506No Hit
CTCTAACCCCACCCACGGGAGCCTGGAGCTGCAGGATCCCAGGGGAGGGG18160.19649936808573257No Hit
TCCTCAGACTGGAGCCCGCTGATGGCCAGGGAGGCTGAGGTGCCAGACTT16980.18373123734007374No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA16360.17702255847371065No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG16270.17604871799310953No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC15720.1700974706116584No Hit
GTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGGTTCTCTGGCTCCAAG15090.1632805872474507No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG15080.16317238274960613No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT14080.1523519329651495No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC14080.1523519329651495No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG13530.14640068558369834No Hit
GTGTTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT13500.14607607209016465No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC13490.14596786759232008No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13440.14542684510309725No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT13430.14531864060525268No Hit
GTACATGGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAAT13410.14510223160956354No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13410.14510223160956354No Hit
GTTCATAGGTTCCCAACTCTAACCCCACCCACGGGAGCCTGGAGCTGCAG13300.1439119821332733No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13290.14380377763542876No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC13180.14261352815913853No Hit
GTATCAACGCAGAGTACATGGGGAGCTTCAGCTGTGGGTAGAGAAGACAG13060.14131507418500372No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12660.13698689427122107No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA12560.1359048492927754No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12370.13384896383372866No Hit
CCTCAGGGGTCCCTGACCGGTTCTCTGGCTCCAAGTCTGGCACCTCAGCC12340.13352435034019494No Hit
CTGTAAAGTGGTACCAGCAGCTCCCAGGAACGGCCCCCAGACTCCTCATG12340.13352435034019494No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA12320.13330794134450583No Hit
GAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACGCTGAGGGTGG12170.1316848738768373No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT12100.13092744239192536No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT12040.13027821540485793No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG12020.1300618064091688No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG11730.1269238759716764No Hit
GTTTAGTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGGTTCTCTGGCT11710.12670746697598725No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGGCTCCAGTCTGAGGATG11640.12595003549107528No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT11280.12205467356867092No Hit
GGTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAA11280.12205467356867092No Hit
GACTGGAGCCCGCTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC11020.11924135662471216No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCA10890.11783469815273281No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC10880.11772649365488824No Hit
GACATAACCATCCAAGGTGGCATCCCATGTTGCACAGTGATAATCAGCCT10600.11469676771524039No Hit
GTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCACTTTACAGTGTTACTT10580.11448035871955126No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC10540.114047540728173No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCC10460.11318190474541645No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCATCCAAG10450.1130737002475719No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGT10310.11155883727774797No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA10220.11058499679714687No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC10120.10950295181870119No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG10030.10852911133810009No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG9990.10809629334672184No Hit
CTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACC9900.10712245286612074No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAACCGGTC9880.10690604387043161No Hit
GTGCAACATGGGATGCCACCTTGGATGGTTATGTCTTCGGAACTGGGACC9880.10690604387043161No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC9880.10690604387043161No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG9850.10658143037689792No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGC9830.10636502138120878No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG9690.10485015841138486No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC9630.10420093142431744No Hit
TTACTAAACATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCA9480.10257786395664896No Hit
GTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAG9430.10203684146742611No Hit
ACTCTAACCCCACCCACGGGAGCCTGGAGCTGCAGGATCCCAGGGGAGGG9390.10160402347604786No Hit
GTGCCAGACTTGGAGCCAGAGAACCGGTCAGGGACCCCTGAGGGCCGCTG9360.10127940998251414No Hit
GTTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGGC9340.10106300098682502No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG9300.10063018299544677No Hit
ATGTTGCACAGTGATAATCAGCCTCATCCTCAGACTGGAGCCCGCTGATG9300.10063018299544677No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC9290.10052197849760218No Hit
GCCTCATCCTCAGACTGGAGCCCGCTGATGGCCAGGGAGGCTGAGGTGCC9280.10041377399975762No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)