FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96688
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG29913.0934552374648354No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA25782.6663081251034253No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG20122.0809200728115176No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT20102.0788515637928184No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG16081.6630812510342545No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT12731.3166059904021181No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT11861.2266258480886976No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG11571.1966324673175575No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT10361.0714876716862487No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT10131.0476998179712065No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCT9751.008398146615919No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTT7780.8046500082740361No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA7350.7601770643720007No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC7200.7446632467317558No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC7030.7270809200728116No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACAT6580.6805394671520768No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGA6230.6443405593248386No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACAT5930.6133129240443488No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC5520.5709084891610128No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT5360.5543604170114182RNA PCR Primer, Index 46 (95% over 23bp)
TCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC5180.5357438358431243No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT5160.533675326824425No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT5100.527469799768327No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC4830.4995449280158861No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTT4770.49333940095978823No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC4590.47472281979149433No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT4500.4654145292073473RNA PCR Primer, Index 46 (95% over 21bp)
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC4400.45507198411385075No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCT4240.43852391196425616No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACAT4110.4250786033427106No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC3930.4064620221744167No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC3920.40542776766506705No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC3860.39922224060896905No Hit
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA3670.37957140493132546No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACA3530.36509184180043025No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC3420.353715042197584No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCT3410.3526807876882343No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC3380.34957802416018535No Hit
CGTCAGATGTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTT3280.3392354790666887No Hit
GACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAT3270.3382012245573391No Hit
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA3230.33406420651994045No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGA3230.33406420651994045No Hit
GATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACA3010.3113106073142479No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTC2990.30924209829554855No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG2860.29579678967400297No Hit
GATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGA2710.28028297203375807No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCT2600.2689061724309118No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTG2590.2678719179215622No Hit
CATCCATAGCGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA2550.2637348998841635No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA2510.25959788184676486No Hit
GATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACA2500.2585636273374152No Hit
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACAT2470.2554608638093662No Hit
CATCCATAGCGTCCTCAGCTGTCTCTCTGTCTCTTATACACATCTCCGAG2440.2523581002813172No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGT2370.24511831871586962No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG2360.24408406420651993TruSeq Adapter, Index 4 (95% over 23bp)
CATCCATAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGG2330.24098130067847098No Hit
ATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA2280.23581002813172267No Hit
CATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG2240.23167301009432403No Hit
CCCGATGAGCAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT2140.2213304650008274No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT2090.2161591924540791No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA2060.2130564289260301No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACA1960.20271388383253353No Hit
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACTA1920.19857686579513484No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACAT1900.19650835677643552No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT1860.1923713387390369No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTA1820.18823432070163826No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGT1780.1840973026642396No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA1750.18099453913619062No Hit
GTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACAT1740.17996028462684097No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC1730.1789260301174913No Hit
CATCCATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAA1720.17789177560814165No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGGCGT1690.17478901208009268No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG1660.17168624855204367No Hit
CTAGTTTCCTCTGGCTTCCTGTCTCTTATACACATCTCCGAGCCCACGAG1650.17065199404269402No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCT1630.1685834850239947No Hit
GTCCAGGGTGGACATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG1620.16754923051464504No Hit
GTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCT1610.16651497600529538No Hit
GCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGA1570.16237795796789675No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATAC1530.1582409399304981No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCTGTCTC1530.1582409399304981No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGG1520.15720668542114843No Hit
CATCCATAGCGTCCTCAGCTGTCTCTGTCTCTTATACACATCTCCGAGCC1510.15617243091179878No Hit
GTATAAGAGACAGCATCCGCAGCGTCATCAGCTGTCTCTTATACACATCT1490.15410392189309946No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACAC1460.1510011583650505No Hit
GAGATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCAC1460.1510011583650505No Hit
TCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC1450.14996690385570083No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCT1450.14996690385570083No Hit
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGA1400.1447956313089525No Hit
GTCCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG1360.14065861327155388No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATTCCGT1300.1344530862154559No Hit
CATCCATAGCGTCCTCATCCTGTCTCTTATACACATCTCCGAGCCCACGA1300.1344530862154559No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCT1280.1323845771967566No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCATCAGCTGTCTCTT1280.1323845771967566No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCT1270.13135032268740693No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTCTGTCTCTTATACACATCTCCGAG1270.13135032268740693No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC1240.12824755915935793No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACA1240.12824755915935793No Hit
GAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG1230.12721330465000827No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC1230.12721330465000827No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGGCGT1230.12721330465000827No Hit
GATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACA1220.1261790501406586No Hit
ATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA1220.1261790501406586No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT1200.12411054112195928RNA PCR Primer, Index 46 (96% over 26bp)
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT1200.12411054112195928No Hit
CCCGATGAGCAGCACTACCTCTGTCTCTTATACACATCTCCGAGCCCACG1180.12204203210325996No Hit
CATCCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAA1170.1210077775939103No Hit
CATCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATC1160.11997352308456065No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATTCC1160.11997352308456065No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTATGCCGT1140.11790501406586133No Hit
CATCCGCAGCGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA1120.11583650504716202No Hit
CCCGATGAGCAGCACTACCTGTCTCCTGTCTCTTATACACATCTCCGAGC1100.11376799602846269No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACA1090.11273374151911301No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGGGGTTGGCGG1090.11273374151911301No Hit
CATCCGCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG1060.10963097799106404No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTGT1060.10963097799106404Illumina PCR Primer Index 8 (95% over 21bp)
GACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCC1050.10859672348171438No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTG1040.10756246897236472No Hit
GGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA1040.10756246897236472No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCTGTCT1030.10652821446301505No Hit
GCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGA1020.1054939599536654No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGGC1010.10445970544431574No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGGGGGTGGGGG1000.10342545093496608No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCC990.10239119642561642No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGTCTCT990.10239119642561642No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCTGTCTC990.10239119642561642No Hit
CGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA990.10239119642561642No Hit
GGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGC980.10135694191626676No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)