FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189311
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA18380.9708891717861086No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT14930.7886493653300652No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG9640.5092149954307991No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG7690.40620988743390507No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA7630.4030404994955391No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG5940.31376940589823094No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG5910.31218471192904795No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC5580.29475307826803515No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC3840.20284082805542203No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT3640.19227620159420214RNA PCR Primer, Index 17 (95% over 21bp)
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC3360.1774857245484943No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAA3210.16956225470257935No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT2900.15318708368768852RNA PCR Primer, Index 17 (95% over 23bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT2740.1447353825187126No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCCGT2730.1442071511956516No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC2520.1331142934113707No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC2470.13047313679606573No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT2460.12994490547300475No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC2350.12413436091933379No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC2240.11832381636566285No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT2190.11568265975035788No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC2130.1125132718119919No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTT2080.10987211519668694No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTC2070.10934388387362594No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC2020.10670272725832096No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT1940.10247687667383301No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCT1920.10142041402771101No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)