FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002295363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485119
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT80651.6624786907954543No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCT62291.2840148499646478No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTCCGAGCCCACGAG44860.9247215631628528No Hit
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG43120.8888540749795412No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATC35940.7408491524759905No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG32050.6606626415374373Illumina Paired End PCR Primer 2 (96% over 29bp)
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG31470.648706812143No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATC29800.6142822688866031No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTCCGAG23530.48503563043294534No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT22080.45514605694685223No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCCTGTCTCTTATACACATCTCCGAGCCCACG21930.4520540321034633No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC20720.4271116983667925No Hit
GAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACG20160.41556813895147376No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTC20160.41556813895147376No Hit
TATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTC19980.411857709139407No Hit
ACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTCCGAGC17870.3683632263424026No Hit
GTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA17670.36424052655121736No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTCTGTCTCTTATACACATCTCCGAGC17660.36403439156165807No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT17350.3576442068853209No Hit
GGTCTGCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACA15670.3230135286393648No Hit
GACAGTCGCTACGGGTCTGCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACC15310.3155926690152313No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTCCGAGCCCAC15240.3141497240883165No Hit
CACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGC15170.31270677916140166No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT14300.2947730350697458No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA14090.29044420028900125No Hit
GCTGGAGGTCGACCAGATGTCCGAAAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13450.27725156095720843No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATCTGTCTCTTATACACATCTCC12720.26220370671938226No Hit
ATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTCC12700.2617914367402637No Hit
ATGCTGGAGGTCGACCAGATGTCCGAAAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12590.25952395185511185No Hit
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT12460.25684419699084143No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAC12460.25684419699084143No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA12080.24901106738758946No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCTGTCTCTTATACA11900.2453006375755227No Hit
CAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATC11550.23808591294094852No Hit
GAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC11100.22880983841078167No Hit
CCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAG10960.225923948556952No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTCCGA10860.22386259866135938No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA10820.22303805870312232No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAC10650.21953376388061485No Hit
GTATCAACGCAGAGTACATGGGGGTGTGGGTCTTGTCGCAGCTCTTGGGCTCGCTTCCGCTCTGGAAGTACAGGT10170.20963928438177026No Hit
GGTCTGCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTCCGAGCCC10120.2086086094339739No Hit
GCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA10090.20799020446529612No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG9840.20283682972631456Illumina Paired End PCR Primer 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG9800.20201228976807753Illumina Paired End PCR Primer 2 (96% over 29bp)
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGCTGTCTCTTATACACATCTCCGAGC9450.19479756513350333No Hit
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCTGTCTCTTATACACATCTCCGAGCCC9210.189850325384081No Hit
CTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGA9030.18613989557201427No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTGTCTCTTATACACATCTCCGAGCCCACGAG8910.18366627569730312No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGC8900.18346014070774389No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCCTGTCTCTTATACACATCTCCGAGCCCAC8870.1828417357390661No Hit
GTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC8820.18181106079126977No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTCCGAGCCCA8610.17748222601052527No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCGTAGCGACTGTCC8340.17191658129242515No Hit
CAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCC8140.16779388150123992No Hit
AGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACG8110.16717547653256212No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGCTGTCTCTTATACACATC8030.165526396616088No Hit
CACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG7960.16408345168917318No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGA7920.16325891173093612No Hit
CCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACAC7660.15789940200239527No Hit
GACAGTCGCTACGGGTCTGCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACA7340.1513030823364989No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACA7290.15027240738870257No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC7170.14779878751399142No Hit
TATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTCCGAGC7010.1445006276810432No Hit
CCCAAGAGCTGCGACAAGACCCACACCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCC6860.14140860283765427No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCGTAGCGACTGTC6860.14140860283765427No Hit
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCGTAGCGACTGTCCTGTCTCTTATACA6780.13975952292118016No Hit
CCCCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGA6650.13707976805690975No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTCTGTCTCTTATACACATCTCCGAGCCCACG6610.1362552280986727No Hit
ACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTCCGAGCCCACG6490.13378160822396154No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGA6430.13254479828660595No Hit
ACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACA6360.13110185335969113No Hit
CCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGC6180.1273914235476244No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAG6170.12718528855806513No Hit
GGTCTGCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATAC6050.12471166868335398No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCTGTCTCTTATACACATCTCCGAGCCCACG6040.12450553369379473No Hit
CAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACAT6020.12409326371467619No Hit
GAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACA5920.12203191381908356No Hit
GGTATCAACGCAGAGTACATGGGGGTGTGGGTCTTGTCGCAGCTCTTGGGCTCGCTTCCGCTCTGGAAGTACAGG5900.12161964383996504No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACGAGACACTG5710.11770307903833904No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGCTGTCTCTTATACACATCTCCGAGCCC5630.11605399912186494No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCGTAGCGACTGTCT5620.11584786413230569No Hit
CCTGTACTTCCAGAGCGGAAGCGAGCCCAAGAGCTGCGACAAGACCCACACCCCCATGTACTCTGCGTTGATACC5570.11481718918450938No Hit
CTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA5550.11440491920539084No Hit
CCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACGA5520.11378651423671306No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGCTGTCTCTTATACACATCTCCGAG5380.11090062438288337No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA5370.11069448939332412No Hit
GTACATGGGGGTGTGGGTCTTGTCGCAGCTCTTGGGCTCGCTTCCGCTCTGGAAGTACAGGTTCTCATTCACAGT5310.10945767945596853No Hit
TATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTG5290.10904540947685001No Hit
GAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA5160.10636565461257959No Hit
ACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGA5140.10595338463346107No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATCTGTCTCTTATACACATCTCCGAGCC5120.10554111465434254No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCTGTCTCTTATAC5040.10389203473786844No Hit
CCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG5030.10368589974830919No Hit
ACAGTCGCTACGGGTCTGCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCT5010.10327362976919065No Hit
ATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTCCGAGCC4950.10203681983183507No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)