FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCGGATGTGTTGGTCTCCATGCAAGAACTTGGAAGAAAAACAGGCAGGAATGCATCTGTCTCTTATACACAT114.285714285714285No Hit
TATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCTCTAACTGTCTCTTATACACATCT114.285714285714285Clontech Universal Primer Mix Short (95% over 21bp)
GGCGGGCGGCGTAGAAAACGCAGCGGAGCCAGGTGAAACCAAGGCACCGCCCTGGCTGGCCCCCGACAGTTCCTC114.285714285714285No Hit
GGCCTAGGCCTAGCCTGCCCTTCCTGTATGGGCTCCCAGGCCACAGCCCTGACACAGCTTTGGGGTGGACTATGC114.285714285714285No Hit
GTTTAATGGCCAGTTTAAAACTTATGCTATCTGCGGGGCCATTCGTAGGATGGGTGAGTCAGATGATTCCATTCT114.285714285714285No Hit
GTGTAAAACTGCTTCCATGTCTAAGCAAAGAAAACTGCCTACATACTGGTTTGTCCTGGCGGGGAATAAAAGGGA114.285714285714285No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTGACGCTGCCGACG114.285714285714285No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)