FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTGCTCGGAACCGAGCCTCTGTCTTTCGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG26.451612903225806No Hit
CTTATGAAAACCTTCAGACACAAAGAGAGAATCATGTTATGAACCCCATGTACTCTGCGTTGATACCTGTCTCTT13.225806451612903No Hit
TCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCC13.225806451612903Clontech Universal Primer Mix Short (95% over 21bp)
CATCATCGGTGCTCCACTTCCCAAAGAGCTTGATGTCTGGGGTCTCTGCCACCGCTGGTGCTGCTGTCTCCCACT13.225806451612903No Hit
TCTTAGAAGCCAGGACCCATGTACTCTGCGTTGATACCTGTCTCTTATACAAATCTCCGAGCCCACGACACTACG13.225806451612903No Hit
GCTCGGAACCGAGCCTCGGTCTTTCGGCCGCCGCCCCAGGTACGCTGCGTTGCTAGCTGTCTCGTAGACACATCT13.225806451612903No Hit
AGGTGTAGCAGTCATCGATACCAGCCATCATGAGCAGCTTCTTAGGCACAGGTGCGGAGACGATGCCAGTGCCCC13.225806451612903No Hit
AGGTGGCAATTCACCGAATTCGAATCACCCTAACAAGCCGCAACGTAAAATCCTTGGAAAAGGTGTGTGCTGACT13.225806451612903No Hit
CCGGCGGCCAGACCCATCCGGTGATCGGCCTCTGGAGCGTGCGCCTGCGGAACGAATTGCGCCATGCGCTGGTCG13.225806451612903No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCT13.225806451612903No Hit
CATTTTAGAAGTGCAGTTCAGCAAGAAGATGGAGCTGTTAGAAGATGCATTGGTGCTCAGAAGCGCAAGAACCTC13.225806451612903No Hit
GGTCAGCGCAGGCGCGCCCCTGACGCGGACCGCGCCGCCACTTCCGCCCCGAGCGAGAGGCTAGAGGCGAGAGGC13.225806451612903No Hit
GATATGCAGGGAGCTGCACCTTCACGATACGGGCCACGCTCTCCAGCACCATCCTGGCCAGCGGCGTCCACTCCT13.225806451612903No Hit
GTACATGGGGAGGGGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCAAAACTGTCTCTTATACACATCTCCG13.225806451612903No Hit
TCTTCACTGTCTGTGTCTTTCATTTTTCTTGCCATCATTGTCAGAAATTCAGGGAAGTCAATTGTGCCATTACCT13.225806451612903No Hit
GAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCAAAAAAAAAAAAAAAATAAAATAA13.225806451612903No Hit
GCTCGGCACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGATGTGAGACCTGTCTCTTATACACATCT13.225806451612903No Hit
GCGTGCTGACGATCTCATACGGTTGTTAGCTCAACGCATGAGCATCTCCACTCCTTGCGATCCTTTACTGAGAAG13.225806451612903No Hit
GTATCAACGCAGAGTACATGGGAGGAATGTACTACGAGTGATAGTCGCACCAGAGACTATAACACTAGACCGAGC13.225806451612903No Hit
GAGCAGGGACAGTCCAGTCCAAAGGAAGCCCCTGGGAAGCTTGGGGGCCGAAGGGTGGAGGGTCCGGCAGGGGGT13.225806451612903No Hit
GTATCAACGCAGAGTACATGGGTGTAACGTCAATTGCTGAGAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAA13.225806451612903No Hit
GTGGTGGTCATGGTCCTGGAGGTGGCAAGAAGGATGACAAGGACAAGAAAAAGAAATATGAACCTCCTGTACCAA13.225806451612903No Hit
GTCGCAGCTCTTGGGCTCGCTTCCGCTCTGGAAGTACAGGTTCTCATTCACAGTCAGAAGGGTCCCGCCTCCTTC13.225806451612903No Hit
ATACACATCTCCGAGCCCACGAGACACCTGAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG13.225806451612903RNA PCR Primer, Index 23 (96% over 28bp)
GTATCAACGCAGAGTACATGGGATGGGGGGACCATCCTCCAAGGCTAAATACTCGTAATCTGTCTCTTATACACA13.225806451612903No Hit
GAGATAGTGTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTCTCTCATATACACATATACAAGCCCA13.225806451612903No Hit
GTCTGGAGTTACGCTGTTCTTTATGTATTGAGAGAACTGTTTCTTGTAAGCATCTTCATCTTCTTCCATTAAGTA13.225806451612903No Hit
GTCTTGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCACGATCTCAGCTCACTGCAACCTCTGCCTCCCAGGTTCAA13.225806451612903No Hit
GGTATCAACGCAGAGTACATGGGAGGGAACGAGTGACAGCATATATCTGATTGACTAGAAGTGAGACTGAGTCTA13.225806451612903No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATT13.225806451612903No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)