FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCTGTCTCTTATACACATCTGACGCTGCCGACGAATTAGACGG26.451612903225806No Hit
GCTAGAAAAGAACATTAAAATACTGCTTCCCAGAAGCACAATAACTCATCTATCATAAACTCTAAATAATCAACC13.225806451612903No Hit
GACAGTCGCTACGGGTCTGCTCGGAGCCGCGCCTCTGGCTTTGGGCCGGCGCCCGCGGGACTCTGCGTAGCATCG13.225806451612903No Hit
CAGCATTCACAGTCAGAAGGAGGCGGGACCCTTCTGACTGTGAATGAGAACCTGTACTTCCAGAGCGGAAGCGAG13.225806451612903No Hit
GTGTATAAGAGACAGTATCAACGCAGAGTACATGGGACACTGCTCAGGGAAGAGCCTGCTACGGTGGACTGTGAG13.225806451612903No Hit
ATATCAAAGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGACTGTCTCTTATACACATCTGACGCTGC13.225806451612903Clontech Universal Primer Mix Short (95% over 21bp)
GACTGTTTGGATGAACACAAAAAGAGCAAAAGCAGTCACAGAATCTGGGTTCAAGACTCCATGGTGAATCCCAGC13.225806451612903No Hit
GTAATGGCACAATTGACTTCCCTGAATTTCTGACAATGATGGCAAGAAAAATGAAAGACACAGACAGTGAAGACT13.225806451612903No Hit
AGTTGGTACAGGAGGTTCATATTTCTTTTTCTTGTCCTTGTCATCCTTCTTGCCACCTCCAGGACCATGACCACC13.225806451612903No Hit
TCAACGCAGAGTACATGGGAAGCACATCATGGGCCAGAATGTTGCAGATTACATGCGCTACTTAATGGAAGAAGA13.225806451612903No Hit
AGTCAGCACACACCTTTTCCAAGGATTTTACGTTGCGGCTTGTTAGGGTGATTCGAATTCGGTGAATTGCCACCT13.225806451612903No Hit
CTCCTGGTCTCCTGCGGGTGGCTCACGGTGCAGGGTACGGCCCATCAGCCCAGATGCTGCATGCCAGACTGAGCA13.225806451612903No Hit
TGCACGGACTCAGAGACGGACAGCCGCGCACGTCTGTGATAAGCTACGGATGGACTCGACAACCAGTAGGCTAGA13.225806451612903No Hit
GTCTGTACCAGGGCGGCGCGTGGTCTACGCCGAGTGACAGAGACGCTCAGGCTGTGTTCTCAGGATGACCGAGTG13.225806451612903No Hit
CTGCGTCAATGACTCCAAGTTCATAGGAGGGTAGAACTGTCGCTTATAACCAGAGTCATATACAGAAGACGGCGT13.225806451612903No Hit
TTTTGGGTCTGCTCGGAACCGAGCCTCTGTCTTTCGGCCCCCTCCCCATGTACCTGTCTCTTATACACATCTGAC13.225806451612903No Hit
GTGGTATCAACGAAGAGTACGTTTGTTTTTGTTTGTTTTTTTTGGTGTGGCTAATTGGGATGTGACGCAGGGGAG13.225806451612903No Hit
ACTCGAAACAGTTCACCCAGAGATATTCTGGATATAGAAACATCACATCTACACAGCGTTGAATCACAGTATCTT13.225806451612903No Hit
GTATCAAATCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCATATACAAATCT13.225806451612903No Hit
GGTACGCCTCATCCCTGCACCCAGGGGCACTGGCATCGTCTCCGCACCTGTGCCTAAGAAGCTGCTCATGATGGC13.225806451612903No Hit
CTCGTGCCCTGTCCGCAGGGTCCCTCCCGCCTCCCCCGCCTCTCGCCTCTAGCCTCTCGCTCGGGGCGGAAGTGG13.225806451612903No Hit
GTATCCGCAAGTGGAACCACCCACCTGTCAACTAAGACTGTCACTTAAACAAATCAGACGCTGCCGACGACGTAG13.225806451612903No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13.225806451612903No Hit
ATTACGAGTATTTAGCCTTGGAGGATGGTCCCCCCATCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA13.225806451612903No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCACGGA13.225806451612903No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT13.225806451612903No Hit
GTCCAGCGATCGATCTTGCGCACATCCTCCTCGACCAGCGCATGGCGCAATTCGTTCCGCAGGCGCACGCTCCAG13.225806451612903No Hit
GTATCAACGCAGAGTACATGGGGTTCATAACATGATTCTCTCTTTGTGTCTGAAGGTTTTCATAAGCTGTCTCTT13.225806451612903No Hit
GGAGAGGAGTGGACGCCGCTGGCCAGGATGGTGCTGGAGAGCGTGGCCCGTATCGTGAAGGTGCAGCTCCCTGCA13.225806451612903No Hit
TTTTTTTTTTTTTGGGTCTGCACGGAACCGAGCCGCGGTGTTTCGGCCTCCTCCCCATGTACGCCGGGGGCGTAT13.225806451612903No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)