FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203088
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG8510.41903017411171517TruSeq Adapter, Index 11 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG3820.18809580083510596TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG3480.17135428976601277RNA PCR Primer, Index 11 (96% over 28bp)
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT2140.10537304025841014No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)