FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98
Sequences flagged as poor quality0
Sequence length75
%GC64

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG1414.285714285714285No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCCGTCCAGCACGGCCC77.142857142857142No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAT77.142857142857142No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGGGGCCCGCGCCCC77.142857142857142No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGA55.1020408163265305No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCCGTCCAGCACGGCCC44.081632653061225No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTAT33.061224489795918No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACTCTTAACGGCCCC22.0408163265306123No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT22.0408163265306123No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAATCGGCACCCTTTCAACAACTCCTGTT11.0204081632653061No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAATCAGCACCCTTTCNACAACTCCTGTT11.0204081632653061No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTCGAGCAGCGTGAAGATGCCGAT11.0204081632653061No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAACTCGTATGCCGGGTTCT11.0204081632653061No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCGGTCCAGCACGGCCC11.0204081632653061No Hit
CCTTTAACAAGTATGCCTGTCAGCGCCACACTGGTGGCCACTTCTGCAATCAGCACCCTTTCNACAACTCCTGTT11.0204081632653061No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTGGAGCAGCGTGAAGATGCCGAT11.0204081632653061No Hit
GCACAGGTCGTATGCGCATTGGCGGAAGTACTGAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCGG11.0204081632653061No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGATGCTGCGCGGGGCCCGCGCCCC11.0204081632653061No Hit
GTCATTGTCACTGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGG11.0204081632653061No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGTGGCGGGAGGCTGCGCGGGGCCCGCGCCCC11.0204081632653061No Hit
TAAAATAGTAGCCCTCCCGGGTGCGGGGGGCCCCCTCAGTGAGGGAGGACAGCCAGAGAAGCTCGGGTGCCAGGT11.0204081632653061No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT11.0204081632653061No Hit
GTAGTGCTGCTCATACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAT11.0204081632653061No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCTCCCCCTGCACCAGGGGAAGCAGTGG11.0204081632653061No Hit
CCTTTAACAAGTATGCCTGTCAGCAACACACTGGTGGCCACTTATACAATCAGCACCCTTTCAACAACTCCTGTT11.0204081632653061No Hit
GCACAGGTCGTATACGCATTTGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGTGAAGGGGCCCTG11.0204081632653061No Hit
CGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTATT11.0204081632653061No Hit
GGCTGTGGTGTGAGTCAGGGTGGCGGCCGTAGCGCGCCATTTGCACACGCAGATCGCGGCAGTCCAGCACGGCAC11.0204081632653061No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCTCCCTCTGCACCAGGGGAAGCAGTGG11.0204081632653061No Hit
GCACAGGTCGTATACGCGTTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG11.0204081632653061No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGAAGGTCGCGAAGGGGCCCTG11.0204081632653061No Hit
GTTTCCAGTTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGA11.0204081632653061No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTGTCTCTCTTCTTTTTTCTTCNTGGCAGTATTAT11.0204081632653061No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCGTG11.0204081632653061No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGGGTAGAGCAGCGTGAAGATGCCGAT11.0204081632653061No Hit
GACTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCCGTCCAGCACGGCCC11.0204081632653061No Hit
CATAGTTACCTTCTCCGAGCACACGAGACAAGAGGCAAGCTCGTATGCCGTCTTCTGCTTCGAAAAAAAAGCCCC11.0204081632653061RNA PCR Primer, Index 15 (96% over 25bp)
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGTCAGGTGGGGCGGGAGGCTGCGCGGGGCCCGCGCCCC11.0204081632653061No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAATCAGCACCCTTTCAACAACTCCTGTT11.0204081632653061No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTCCTCAGAGGGACTCAGCACCGCCTGGCAGTTCTCGAAGGGTCCCTG11.0204081632653061No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGTGGCGGGAGGTTGCGCGGGGCCCGCGCCCC11.0204081632653061No Hit
GCACAGGTCGGATACGCAGTGGCGGAAGTACTCAGAGGGACTCAGCAGCGCATGGCAGGTCGCGAAGGGGCCCTG11.0204081632653061No Hit
GGCTGTAGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGCATCCCGCGGCCGTCCAGCACGGCCC11.0204081632653061No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACNCTTAACGGCCCC11.0204081632653061No Hit
GCACAGGTCGTATACGCATTGGCGGAAGGACGCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG11.0204081632653061No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCTTGAAGATGCCGAT11.0204081632653061No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCGCATTGACTACCTGA11.0204081632653061No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACATG11.0204081632653061No Hit
TCTCATGCTCGCAGATGCCGCCCAGCGACTCCGGGGCGTCAGGTGTAGCGGTAGGCTTCGCTGGGCCCACTCCCC11.0204081632653061No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCGGTCCAGCACGGCCC11.0204081632653061No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTTTAGAGCAGCGTGAAGATGCCGAT11.0204081632653061No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCTCATTGACTACCTGA11.0204081632653061No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAGTGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG11.0204081632653061No Hit
CAGTGACATGTATTCACCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTAT11.0204081632653061No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTCGGTCACTGTCGCTCTGTCCACTCTTAACGGCCCC11.0204081632653061No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGGGGCCAGCGCCCC11.0204081632653061No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)