FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002295425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG514.705882352941178No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAT411.76470588235294No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACATG38.823529411764707No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGGGGCCCGCGCCCC25.88235294117647No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAAAAAACGGAGTGGTGCCATTCAGGTA12.941176470588235No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT12.941176470588235No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAAGGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAT12.941176470588235No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCCCCGCCTGGCCGGTCGCGCAGGGCCCCTG12.941176470588235No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCGGAGGGACTCAGCACCGCCTGGCAGGGCGCGAAGGGGCCCTG12.941176470588235No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAGCAGCGCTCTGCACAGCATCATCCAT12.941176470588235No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCCGTCCAGCACGGCCC12.941176470588235No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT12.941176470588235No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCACTCAGCACCCTTTCAACAACTCCTGTT12.941176470588235No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCTCCCCCTGCACCAGGGGAAGCAGTGG12.941176470588235No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAATCAGCACCCTTTCAACAACTCCTTTT12.941176470588235No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCCATTGGGATCATG12.941176470588235No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG12.941176470588235No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTAT12.941176470588235No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATTATTCTTTTATTGGCATGTGACAATA12.941176470588235No Hit
CCTTTAACAAGTATGCCTGTCAGCAACACACTGGTGGCCACTTCTACAATCAGCACCCTTTCNACAACTCCTGTT12.941176470588235No Hit
ACATCAGCCTCTTTATTCTCAGCATGGACAAAAGCCACCGCAGCAATAAGCTGGAGGACCGCATTGACTACCTGG12.941176470588235No Hit
CTTCTTGTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACTCTTAACGGCCCCC12.941176470588235No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATAACAGTTGCTTTGTTGACAACAGTCA12.941176470588235No Hit
CAGTGACATGTATTCCCACTCTTACCTTAAGGCTGTGAGTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTAT12.941176470588235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)