FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG1088.06572068707991No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG886.572068707991038No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT795.8999253174010455No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG755.6011949215832715No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC725.37714712471994No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT614.5556385362210605No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG614.5556385362210605No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC554.1075429424943986No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC533.9581777445855115No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC513.8088125466766245No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG483.5847647498132935No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA463.4353995519044065No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT423.136669156086632No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG413.061986557132188No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC392.912621359223301No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT382.8379387602688575No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA292.165795369678865No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT261.9417475728155338No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG251.8670649738610903No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG231.7176997759522032No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT211.568334578043316No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAG201.4936519790888723No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATA141.0455563853622107No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTT141.0455563853622107No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCT90.6721433905899925No Hit
GTTTCCAGGTCAAGGGTAACGGAACGTGGACCAAGTCCCGCGTCTCCTCC90.6721433905899925No Hit
CCTGACTCACGTTTCCTTATTGCTGGGATGTTCACAGAGCCAACGTCCTG90.6721433905899925No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATT70.5227781926811054No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAACCAC50.37341299477221807No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGACACCGGGTCATTCTTTT40.2987303958177745No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCAC30.22404779686333084No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCG20.14936519790888725No Hit
CATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAA20.14936519790888725No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTATCTGTG20.14936519790888725No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGATCG20.14936519790888725No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAAACCA20.14936519790888725No Hit
CAGTGACATGTATTCCCCCTCTTACCTTCAGGCTGTGATTCTTTCTCTCT20.14936519790888725No Hit
CTGGAATCCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCT20.14936519790888725TruSeq Adapter, Index 1 (95% over 23bp)
CTGTTTTCATCCCACAGGTTGTTATTTTGTACTTTGAGCCAAGCACATTT20.14936519790888725No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGTGGGGACCG20.14936519790888725No Hit
GAACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGGG20.14936519790888725No Hit
GTAAAAGTTGAAATGTCAGGGCCGCGGGCACGGGGGGCACTGCGTCCTGC20.14936519790888725No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)