FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002295473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361261
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG175384.854661864967434No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA150264.159319716216254No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG147164.073509180343297No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT85212.3586825037853516No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTT71561.9808393377641096No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCT70411.9490063970370453No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG65971.8261035650125532No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT54021.4953177896313192No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC48001.328679265129643No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACAT42571.1783724232618522No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT41951.161210316087261No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT41201.1404497025696103No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC38421.0634970284641851No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC37291.0322177040975915No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT34680.9599707690561671No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT34350.9508360991084008No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC33680.9322899510326329No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACA31560.8736066168227403No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT29540.8176913644152012No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC29180.8077262699267288No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC24220.6704294125299991No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACAT23970.6635092080241155No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC23470.6496687990123484No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT22890.6336139245586986No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC21480.5945839711455153No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA20260.5608133731568036No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGT20260.5608133731568036No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT19200.5314717060518572Illumina PCR Primer Index 7 (95% over 22bp)
GATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACA18840.5215066115633848No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC18410.5096038598132652No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC17300.4788781518071422No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC17150.4747260291036121No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTT16920.46835944095819915No Hit
TCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC16300.451197333783608No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT15030.41604269489371953No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTA14960.4141050376320721No Hit
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA14430.39943420407959895No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTG13600.3764591251200656No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG13470.3728606187770061No Hit
TCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC12230.3385364044278237No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCT11860.32829450175911595No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA11830.32746407721841No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT11650.32248152997417384No Hit
GATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACA11520.31888302363111437No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCT10470.2898181647064034No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTC10190.2820675356598138No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACAT10040.2779154129562837No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTC10030.27763860477604835No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA9900.2740400984329889No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGT9750.2698879757294588No Hit
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA9730.2693343593689881No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCT9320.25798522397933904No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA8450.23390291229886426No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACA8120.22476824235109796No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTC8080.22366100963015662No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTC8030.22227696872897987No Hit
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACAT7880.21812484602544974No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCT7710.21341910696144892No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCT7520.20815975153697744No Hit
CATCCATAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAC7480.20705251881603606No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCT7420.205391669734624No Hit
GATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGA7330.2029003961125059No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATAC7190.19902508158921112No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG6920.19155126072285686No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACAC6580.18213978259485525No Hit
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTG6440.17826446807156043RNA PCR Primer, Index 41 (96% over 27bp)
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGA6410.17743404353085443No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCT6250.17300511264708895No Hit
GCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGA6210.17189787992614758No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTCTGTCTCTTATACACATCTCCGAG6190.1713442635656769No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCTGTCTC6150.17023703084473552No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACA6070.16802256540285276No Hit
CATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGT6040.16719214086214676No Hit
GCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGA5980.1655312917807347No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTA5850.16193278543767525No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCCTGTC5790.16027193635626322No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGTCTCT5730.15861108727485115No Hit
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTC5700.15778066273414512No Hit
CATCCATAGCGTCCTCAGCTGTCTCTCTGTCTCTTATACACATCTCCGAG5460.1511372664084969No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCCTGTCTCT5390.14919960914684952No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG5360.14836918460614348No Hit
GAGATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCAC5330.14753876006543745No Hit
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACTC5100.1411721719200246No Hit
ATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG5030.1392345146583772No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATAC4970.13757366557696513No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCC4960.1372968573967298No Hit
CCCGATGAGCAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT4950.13702004921649444No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATA4880.13508239195484706No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCCTGTC4780.13231431015249362No Hit
ATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCC4780.13231431015249362No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTTTGCCGT4740.13120707743155224No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACAC4710.1303766528908462No Hit
CATCCATAGCGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTC4630.1281621874489635No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCTGTCT4570.12650133836755145No Hit
GTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTG4500.12456368110590404No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTGTCTCTTATACACATCTCCGAGCC4460.12345644838496267No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATA4430.12262602384425665No Hit
CATCCATAGCGTCCTCAGCTGTCTCTGTCTCTTATACACATCTCCGAGCC4390.12151879112331526No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC4360.12068836658260926No Hit
GTCCAGGGTGGACATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG4270.11819709296049118No Hit
ATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACAT4240.11736666841978514No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCCTGTCTCTTATACACATCTCC4200.11625943569884378No Hit
CATCCGCAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAC4190.11598262751860842No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGTCTCT4100.11349135389649034No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC4060.11238412117554898No Hit
CATCCGCAGCGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTC4030.11155369663484295No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGTCTTCT4000.11072327209413692RNA PCR Primer, Index 7 (95% over 21bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATCTGTCTCTTATACACAT3980.11016965573366624No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC3960.10961603937319556No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCTGT3900.1079551902917835No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACA3900.1079551902917835No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTTTGCCGT3890.10767838211154816No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGTCTT3820.10574072484990077No Hit
AGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAG3800.10518710848943008No Hit
CGTCAGATGTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTT3780.1046334921289594No Hit
ATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA3770.10435668394872405No Hit
AGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCG3750.10380306758825336No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACA3750.10380306758825336No Hit
GACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCC3710.102695834867312No Hit
GAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGC3630.10048136942542925No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)