FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002295486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1592170
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGC100610.6319048845286622No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG41070.2579498420394807Illumina PCR Primer Index 11 (96% over 29bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC37710.2368465678916196No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC36170.22717423390718328No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGC29930.18798243906115553No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC29080.18264381316065495No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC24650.15482015111451666No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCA23350.14665519385492756No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTG20530.1289435173379727No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)