FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002295522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814663
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGC373364.5829993506517415No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG351084.309512031355297Illumina Paired End PCR Primer 2 (96% over 29bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC240862.9565599517837438No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC197202.4206328260888244No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG163992.0129796001536833RNA PCR Primer, Index 5 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG143381.7599915547901401Illumina Paired End PCR Primer 2 (96% over 29bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGA115691.4200964079625562No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG110401.3551615821511471Illumina Paired End PCR Primer 2 (96% over 29bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG84381.0357657092564654No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC82551.0133024330305904No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTG80270.98531540035573No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTG78260.9606426215502607No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG76540.9395295969008044Illumina Paired End PCR Primer 2 (96% over 29bp)
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGAT75260.923817578557023No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAG70780.868825514353788No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC69290.8505357430004798No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAG69220.8496764919973044No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCG63280.7767629068706937No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC62310.764856142969547No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCG59390.7290131011227955No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG54770.6723025349132096TruSeq Adapter, Index 5 (96% over 28bp)
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGC53730.6595365200088872No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCG53540.6572042672859821No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGAT52110.6396509967925388No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG46180.5668601618092389No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGAT45640.5602316540704562No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGC42860.5261071142300559No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG41870.5139548500422874TruSeq Adapter, Index 1 (96% over 26bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA41580.5103950958862745No Hit
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCG36740.45098402652385094No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG36610.44938827466081066TruSeq Adapter, Index 5 (96% over 28bp)
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACA34060.4180869881165586No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG34050.4179642379732478No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGAT33500.41121298009115426No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCC29520.36235842305345894No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGA29360.3603944207604863TruSeq Adapter, Index 5 (96% over 28bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTC29210.35855316861082437No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATC28560.35057440929562284No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG28300.34738290556954227No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCG27620.3390358958244084No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCAC27620.3390358958244084No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA25840.31718637031508734No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCT25440.3122763645826557No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGC24990.30675260813367No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATG24230.2974235972420498No Hit
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT23840.2926363416529289No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC23510.28858558692367275No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGAT23150.2841665817644842No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG23140.2840438316211734RNA PCR Primer, Index 1 (96% over 26bp)
GATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGA23130.2839210814778626No Hit
CCCGATGAGCAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC22850.28048407746516046TruSeq Adapter, Index 5 (95% over 22bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA22350.27434657029962084No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGAT21990.2699275651404323No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTC21380.262439806398474No Hit
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCT21100.2590028023857718TruSeq Adapter, Index 5 (95% over 23bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA19620.2408357811757745No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA19090.2343300235803025No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACA18920.23224327114401908No Hit
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTC18920.23224327114401908No Hit
GATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGA18020.22119575824604776No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT17370.21321699893084625TruSeq Adapter, Index 5 (95% over 21bp)
GTCCAGGGTGGACATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC17200.21113024649456277No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT17040.2091662442015901No Hit
GTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCA16870.20707949176530663No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCG16270.19971448316665907No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGA16160.1983642315902404TruSeq Adapter, Index 5 (96% over 28bp)
GCGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGA15620.1917357238514576No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGAT15400.18903522069862017No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT14400.17676020636754095No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCA14350.17614645565098697No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTT14220.17455070378794668No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCTGTCTCTTATACACATCTCCGAGCCCAC13990.17172745049179847No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG13900.17062269920200132Illumina Paired End PCR Primer 2 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG13890.17049994905869054TruSeq Adapter, Index 1 (96% over 26bp)
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA13800.1693951977688934TruSeq Adapter, Index 5 (96% over 28bp)
CATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG13570.16657194447274518TruSeq Adapter, Index 1 (96% over 26bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCG13540.1662036940428128No Hit
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCG13510.1658354436128804No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGA13510.1658354436128804No Hit
CCCGATGAGCAGCACTACCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG13440.16497619260970486No Hit
GGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTG12760.156629182864571TruSeq Adapter, Index 5 (95% over 24bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCT12440.15270117827862564No Hit
CCCGATGAGCAGCACTACCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTA12230.150123425269099No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGAGCC12100.1485276734060587No Hit
GAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGC11700.143617667673627TruSeq Adapter, Index 5 (96% over 25bp)
GCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAA11550.1417764155239651TruSeq Adapter, Index 5 (96% over 28bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG11510.14128541495072197No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT11490.14103991466410037No Hit
GTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACG11390.13981241323099244No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACAT11170.137111910078155No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT11050.13563890835842551No Hit
CGTCAGATGTGTATAAGAGACAGGGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCG10780.1323246544890341No Hit
ATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCG10650.13072890262599382No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGA10650.13072890262599382No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG10360.12716914846998084Illumina Paired End PCR Primer 2 (96% over 29bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTC9990.12262739316748152No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA9590.11771738743504984No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGA9570.11747188714842825No Hit
CCCGATGAGCAGCACTACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT9260.11366663270579369No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC9160.11243913127268576No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG9150.11231638112937498RNA PCR Primer, Index 33 (96% over 27bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACAC9080.11145713012619943No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACG8880.10900212725998358No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGA8880.10900212725998358No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGAGCCCACG8860.10875662697336198No Hit
ACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA8820.10826562640011883TruSeq Adapter, Index 5 (96% over 28bp)
GTCCAGGGTGGACATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT8700.10679262468038932TruSeq Adapter, Index 5 (95% over 21bp)
TCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT8630.10593337367721377TruSeq Adapter, Index 5 (95% over 21bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA8630.10593337367721377No Hit
GTCCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG8540.10482862238741664TruSeq Adapter, Index 5 (96% over 28bp)
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATG8460.1038466212409303No Hit
GTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC8370.10274186995113317TruSeq Adapter, Index 5 (95% over 22bp)
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGAG8230.10102336794478209No Hit
GACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAA8230.10102336794478209TruSeq Adapter, Index 5 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)