FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2017
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT1004.95785820525533No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT502.478929102627665RNA PCR Primer, Index 27 (96% over 25bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC321.5865146256817053No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT271.338621715418939RNA PCR Primer, Index 27 (95% over 23bp)
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG241.189885969261279RNA PCR Primer, Index 27 (100% over 24bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA200.991571641051066No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG110.5453644025780863No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG110.5453644025780863No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG100.495785820525533No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG70.34705007436787305No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC60.2974714923153198No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA60.2974714923153198No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCT50.2478929102627665No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCT50.2478929102627665No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC40.19831432821021316No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC40.19831432821021316No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT40.19831432821021316No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTA40.19831432821021316No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTTTGCCGT40.19831432821021316No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA30.1487357461576599No Hit
CCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACG30.1487357461576599No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCT30.1487357461576599No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT30.1487357461576599No Hit
GACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTAT30.1487357461576599No Hit
CAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC30.1487357461576599No Hit
GGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA30.1487357461576599No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA30.1487357461576599No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTGT30.1487357461576599RNA PCR Primer, Index 27 (95% over 23bp)
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC30.1487357461576599No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATT30.1487357461576599No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT30.1487357461576599RNA PCR Primer, Index 27 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)