FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109355
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG33673.078963010379041No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA26782.448904942618079No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG24972.2833889625531527No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT22962.0995839239175162No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG17721.6204105893649123No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT14711.345160257875726No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT14031.2829774587353115No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG12721.1631841250971606No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT11801.0790544556718942No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT11081.013213844817338No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCT10020.9162818343925747No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTT9610.878789264322619No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC9470.865986923323122No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA8780.8028896712541722No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACAT7950.7269900781857254No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC7870.7196744547574413No Hit
TCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC7040.6437748616889946No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACAT6220.5687897215490832No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT6210.5678752686205477No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGA5860.5358694161218052No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC5800.5303826985505921No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGA5780.5285537926935211No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT5680.5194092634081661No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC5320.486488957980888No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT5260.4810022404096749RNA PCR Primer, Index 46 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT5160.47185771112431985RNA PCR Primer, Index 46 (95% over 21bp)
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTT4850.4435096703397192No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC4810.4398518586255773No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC4700.42979287641168673No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC4430.4051026473412281No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCT4330.39595811805587305No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACAT4260.3895569475561246No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC4240.3877280416990535No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC4240.3877280416990535No Hit
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA4230.386813588770518No Hit
CGTCAGATGTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTT4220.38589913584198254No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC4180.3822413241278405No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC4010.36669562434273695No Hit
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA3870.3538932833432399No Hit
GATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACA3850.3520643774861689No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCT3570.3264596954871748No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG3500.3200585249874263No Hit
CATCCATAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGG3360.3072561839879292No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTC3240.2962827488455032No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACA3180.29079603127429016No Hit
ATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA3180.29079603127429016No Hit
GACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAT3090.2825659549174706No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT3010.2752503314891866No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA2910.26610580220383157No Hit
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACAT2870.26244799048968953No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC2860.26153353756115405No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTG2770.2533034612043345No Hit
CATCCATAGCGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA2740.250560102418728No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGT2730.24964564949019247No Hit
CATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG2560.23409994970508893No Hit
GATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGA2500.2286132321338759No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCT2480.2267843262768049No Hit
CCCGATGAGCAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT2430.22221206163412738No Hit
GATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACA2420.22129760870559187No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG2410.2203831557770564No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA2370.21672534406291435No Hit
GTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCT2350.21489643820584337No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT2300.21032417356316585No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACA2260.20666636184902382No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG2190.2002651913492753TruSeq Adapter, Index 4 (95% over 23bp)
CATCCATAGCGTCCTCAGCTGTCTCTCTGTCTCTTATACACATCTCCGAG2180.1993507384207398No Hit
GTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACAT2160.19752183256366876No Hit
TCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC2150.1966073796351333No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCT2130.1947784737780623No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACAT2130.1947784737780623No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATAC2070.18929175620684924No Hit
GAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG2060.18837730327831376No Hit
CTAGTTTCCTCTGGCTTCCTGTCTCTTATACACATCTCCGAGCCCACGAG2030.18563394449270723No Hit
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACTA2010.18380503863563624No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC1960.1792327739929587No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGGCGT1960.1792327739929587No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTA1930.1764894152073522No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA1930.1764894152073522No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT1880.1719171505646747No Hit
GCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGA1860.17008824470760367No Hit
CATCCATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAA1860.17008824470760367No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGG1810.16551598006492615No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACAC1800.16460152713639067No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATTCC1790.16368707420785514No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGT1700.15545699785103562No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC1690.1545425449225001No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTG1630.14905582735128708No Hit
GTATAAGAGACAGCATCCGCAGCGTCATCAGCTGTCTCTTATACACATCT1580.14448356270860957No Hit
GTCCAGGGTGGACATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG1520.13899684513739655No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCTGTCTC1480.13533903342325454No Hit
GGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA1460.13351012756618352No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGGC1450.13259567463764801No Hit
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGA1450.13259567463764801No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCT1450.13259567463764801No Hit
CATCCATAGCGTCCTCAGCTGTCTCTGTCTCTTATACACATCTCCGAGCC1430.13076676878057703No Hit
GTCCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG1390.127108957066435No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACA1380.1261945041378995No Hit
GAGATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCAC1380.1261945041378995No Hit
ATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA1380.1261945041378995No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCT1380.1261945041378995No Hit
CCCGATGAGCAGCACTACCTGTCTCCTGTCTCTTATACACATCTCCGAGC1370.125280051209364No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCATCAGCTGTCTCTT1370.125280051209364No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGGCGT1350.12345114535229298No Hit
CAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC1340.12253669242375748No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGGGGTTGGCGG1340.12253669242375748No Hit
CGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA1340.12253669242375748No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATTCCGT1330.121622239495222No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACCTGTCTCTTATACACATCT1320.12070778656668647No Hit
CATCCGCAGCGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA1320.12070778656668647No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTATGCCGT1310.11979333363815096No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCT1300.11887888070961547No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT1290.11796442778107996RNA PCR Primer, Index 46 (96% over 26bp)
CATCCGCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG1290.11796442778107996No Hit
GTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGA1280.11704997485254447No Hit
CCCGATGAGCAGCACTACCTCTGTCTCTTATACACATCTCCGAGCCCACG1280.11704997485254447No Hit
GCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAA1260.11522106899547345No Hit
GGTCACGTCCTTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA1260.11522106899547345No Hit
GATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACA1250.11430661606693795No Hit
GCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGA1230.11247771020986695No Hit
CATCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATC1220.11156325728133144No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCTGTCTC1180.10790544556718944No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTA1180.10790544556718944No Hit
CCCGATGAGCAGCCACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC1150.10516208678158293No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCCTGTC1140.10424763385304742No Hit
CAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG1130.10333318092451191No Hit
CCCGATGAGCAGCACTACCCTGTCTCTTATACACATCTCCGAGCCCACGA1120.10241872799597641No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCCTGTCTCTTATACACATCTCC1110.1015042750674409No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATA1100.10058982213890541No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCTGTCT1100.10058982213890541No Hit
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTC1100.10058982213890541No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)