FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002295565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190907
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA55432.903507990801804No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG37091.942830802432598No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG34801.8228771077016557No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG20951.09739297144683No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA20281.0622973489709648No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC17930.9392007626750197No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG15610.8176756221615761No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCT13290.6961504816481323No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC12730.666816827041439No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC11800.6181020077838948No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT11440.5992446583938776No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTT10880.5699110037871843No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC10740.562577590135511No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC10540.5521012849188348No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT9950.5211961845296401No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC8830.4625288753162535No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACAT8550.44786204801290685No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTC7870.4122426102762078No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG7470.39128999984285545No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT7290.3818613251478469No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT7280.38133750988701304No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT7280.38133750988701304No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT6730.3525276705411535No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACAT5960.31219389545695025No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGC5880.3080033733702798No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC5860.30695574284861216No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC5450.285479317154426No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC5310.27814590350275264No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC5300.2776220882419188No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTG5120.2681934135469103No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTA5070.2655743372427412No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA5050.26452670672107365No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTT5040.2640028914602398No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCT4920.2577171083302341No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC4690.24566935733105646No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACAC4590.2404312047227184No Hit
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA4530.23728831315771556No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATAC4470.23414542159271268No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC4440.23257397581021128No Hit
CGTCAGATGTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTT4290.22471674689770413No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC4280.22419293163687032No Hit
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA3930.205859397507687No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACAT3860.20219269068185033No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGGTTGGCGG3670.19224020072600795No Hit
TCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC3650.19119257020434033No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACAC3550.18595441759600226No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACA3350.17547811237932606No Hit
GATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACA3310.17338285133599082No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT3300.172859036075157No Hit
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGA3220.16866851398848653No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCT3190.16709706820598513No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT3180.1665732529451513No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA3170.16604943768431749No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATAC3060.1602874698151456No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACAT3060.1602874698151456No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT3030.15871602403264418No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGT2990.15662076298930894No Hit
CCCGATGAGCAGCCACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC2930.15347787142430608No Hit
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACAT2910.15243024090263846No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCT2870.15033497985930322No Hit
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACTC2860.1498111645984694No Hit
GTCCAGGGTGGACATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG2810.14719208829430036No Hit
GTATAAGAGACAGCATCCGCAGCGTCATCAGCTGTCTCTTATACACATCT2800.14666827303346655No Hit
GATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGA2770.14509682725096512No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGGTTTGCGG2770.14509682725096512No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA2680.14038248990346083No Hit
GTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCT2610.13671578307762416No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCT2600.13619196781679038No Hit
GATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACA2590.13566815255595657No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGC2500.13095381520845228No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCT2470.12938236942595085No Hit
CCCGATGAGCAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT2450.12833473890428324No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTG2440.12781092364344943No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCTGTCTC2420.1267632931217818No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCATCAGCTGTCTCTT2370.12414421681761277No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTA2220.11628698790510564No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCCTGTCTCTTATACACATCTCC2200.115239357383438No Hit
GGTCGGTAGTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCT2190.11471554212260421No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTA2170.11366791160093657No Hit
GGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA2120.11104883529676754No Hit
GTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACAT2120.11104883529676754No Hit
GGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC2060.10790594373176468No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGGGTGGGGG2050.10738212847093086No Hit
CATCCGCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG1970.1031916063842604No Hit
GAGATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCAC1960.1026677911234266No Hit
GCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGA1930.10109634534092517No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGGGGTTTGC1910.10004871481925753No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)